摘要
目的:探讨硫酸多糖在介导胶质细胞源性神经生长因子神经营养活性中的作用。资料来源:应用计算机检索Medline1994-09/2005-02与胶质细胞源性神经生长因子和硫酸多糖相关文献,检索词“GDNF,sulfatepolysaccha-rideandsignaling”,并限定语言种类为English,以及中文全文数据库CNKI1994-09/2005-04期间的文章,限定文章语言种类为中文,检索词“胶质细胞源性神经生长因子,硫酸多糖”。资料选择:对资料进行初审,选出包含研究目的的文献,筛除与目的无关的文献。选择以胶质细胞源性神经生长因子,硫酸多糖为主要研究对象的文章30余篇。内容相似的,以近几年发表在较权威杂志者优先。资料提炼:共找出相关性最强的文献29篇,并查找全文,其中5篇关于硫酸肝素氨基葡聚糖的结构,12篇讲述硫酸肝素氨基葡聚糖介导胶质细胞源性神经生长因子信号转导,5篇关于C-Ret的磷酸化与胶质细胞源性神经生长因子信号转导,7篇关于硫酸肝素活性片段及其修饰与胶质细胞源性神经生长因子信号转导。资料综合:利用所查文献对硫酸多糖在介导胶质细胞源性神经生长因子神经营养活性中的作用进行综合分析。胶质细胞源性神经生长因子的信号转导需要硫酸肝素的介导,而这一过程可以从C-Ret的磷酸化来体现。硫酸肝素寡糖片段的大小及其硫酸化修饰对胶质细胞源性神经生长因子的信号转导至关重要。结论:硫酸多糖可促进胶质细胞源性神经生长因子介导的神经营养作用,其中2-O硫酸化基团的存在尤为重要。
OBJECTIVE： To investigate the effects of sulfate polysaccharide in mediating the neurotrophic function of glial cell line-derived neurotrophic factor （GDNF）. DATA SOURCES： A computer：based online search of Medline database was undertaken to identify articles about GDNF and sulfate polysaccharide published in English from September 1994 to February 2005 by using the keywords of “GDNF, sulfate polysaccharide, signaling”. At the same time, Chinese relevant articles were searched in China National Knowledge Infrastructure （CNKI） published between September 1994 and April 2005, the keywords were “GDNF, sulfate polysaccharide”. STUDY SELECTION： All articles which are related to the objective were selected firstly, and those irrelated to the objective were screened out. More than 30 articles mainly researched GDNF and sulfate polysaccharide were selected, and those published in authoritative journals in recent years were selected superiorly. DATA EXTRACTION： Total 29 articles were closely related to our objective, including 5 about the structure of glycosaminoglycan （GAGs）, 12 related to GDNF signaling transduction induced by heparan sulfate, 5 about C-ret phosphorylation and 7 focused on heparan sulfate oligosaccharides fractions and their modifications with GDNF signaling. DATA SYNTHESIS： The role of heparan sulfate in neurotrophic function and signal transduction of GDNF was summarized and analyzed. Signaling transduction of GDNF required heparan sulfate. The process can be embodied by C-ret phosphorelation. The molecular mass and residue modification of heparan sulfate oligosaccharides were important to GDNF signaling. CONCLUSION： Heparan sulfate can promote obviously the neurotrophic function of GDNF. 2-0 sulfated in oligosaccharides is particularly important.
出处
《中国临床康复》
CSCD
北大核心
2005年第38期126-129,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家重点基础研究发展规划(973)资助项目(2003CB716400)~~
