摘要
目的探讨发育性髋关节脱位(DDH)和雌激素(ER)基因多态性之间的相关性.方法取2003年2月～2004年8月我院治疗DDH患儿90例,设为DDH组,对照组50例.分别提取DDH组与正常儿童DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法研究ER基因限制性内切酶XbaⅠ和PvuⅡ酶切多态性与DDH发生的关系.结果DDH组和对照组雌激素受体PvuⅡ内切酶酶切多态性分布差异存在显著性意义(P＜0.05);DDH组和对照组雌激素受体XbaⅠ内切酶酶切多态性分布差异无显著性意义(P＞0.05).结论雌激素受体PvuⅡ内切酶酶切基因型多态性与DDH的发生之间存在相关性,雌激素受体XbaⅠ内切酶酶切基因型多态性与DDH的发生之间无显著相关性.
Objective To investigate the relationship between developmental dislocation of the hip （DDH） and estrogen receptor gene polymorphism. Methods Xba Ⅰ and Pvu Ⅱ polyrnorphism of estrogen receptor gene was detected by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） in 48 patients with DDH and normal controls. The relationship between the polymorphism of estrogen receptor gene and the DDH was explored using x^2 analysis. A P value less than 0.05 was considered statistical significant difference. Results There was no association between Xba Ⅰ polymorphism of estrogen receptor gene and DDH. Pvu Ⅱ polymorphism of estrogen receptor gene, however, showed a close correlation with DDH. Conclusions The difference in estrogen genotypes among individuals might influence the development of DDH.
出处
《中华小儿外科杂志》
CSCD
北大核心
2005年第11期576-578,共3页
Chinese Journal of Pediatric Surgery
