摘要
采用正交试验设计研究了不同基本培养基、TDZ、生长素种类及其浓度4个因素组合对满园香梨(Pyrusus鄄suriensisMaxim.cv.Manyuanxiang)离体叶片不定芽再生的影响。结果表明,基本培养基的种类是影响叶片不定芽再生最重要的因素,其次是生长素的种类。通过叶片不定芽再生频率和平均每叶再生不定芽数分析表明,NN69+TDZ3.0mg/L+IAA0.2mg/L为满园香梨叶片不定芽发生的最佳培养基组合,在该培养基组合上,满园香梨叶片再生率和平均再生芽数分别达到了74.8%和4.40。同一叶片的基部和顶端比中部更有利于不定芽的再生。
The basal media, TDZ, the types and concentrations of auxin were used to compare their effects on adventitious shoots induction from leaves of pear （Pyrus ussuriensis Maxim. cv. Manyuanxiang） by using the orthogonal experiment design of L16 （45）. The results showed that the basal media played a vital role in inducing the adventitious buds, followed by plant growth regulators through comparison of bud regeneration rates and buds per leaf regenerated. 74.8% regeneration rate and 4.40 buds per leaf were achieved on the medium of NN69＋TDZ 3.0 mg/L＋IAA 0.2 mg/L. The basal and apical leaf sections were more effective than middle leaf sections in inducing adventitious buds.
出处
《果树学报》
CAS
CSCD
北大核心
2005年第6期706-708,F0003,共4页
Journal of Fruit Science
基金
教育部"新世纪优秀人才支持计划"项目(NCET-04-0905)
高等学校全国优秀博士学位论文作者专项基金项目(200253)
农业部"948"项目(2004246)。
关键词
梨
离体叶片
不定芽再生
Pear（Pyrus ussuriensis Maxim. ）
Leaves in vitro
Adventitious bud regeneration
