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酶解-连续流动分析法测定烟草中的淀粉 被引量:12

Determination of Starch in Tobacco with Enzymolysis-Autoanalyzer
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摘要 先采用耐高温淀粉酶于95℃下处理30min,再用糖化酶于55℃下处理30min,然后采用连续流动分析法测定了烟叶样品中的淀粉含量.方法的相对标准偏差(RSD)为2.0%,回收率在96%~100%之间.并采用该法测定了部分烟叶的淀粉含量. The tobacco sample was treated with high-temperature resistant amylase at 95℃ for thirty minutes, during which the starch in tobacco was liquefied into dextrin, then dealt with glucoamylase at 55℃ for thirty minutes, during which the dextrin was hydrolyzed into glucose. The glucose in the resultant sample solution was determined with an autoanalyzer. The results showed that the relative standard deviation of the method was 2.0 %, and the recoveries were from 96% to 100% .The starch in some tobacco leavers were also determined.
出处 《烟草科技》 EI CAS 北大核心 2005年第11期28-29,37,共3页 Tobacco Science & Technology
关键词 淀粉酶 糖化酶 连续流动分析仪 烟草 淀粉 Amylase Glucoamylase Autoanalyzer Tobacco Starch
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