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液体培养条件下细菌浓度两种测定方法比较 被引量:11

Comparison of Two Measuring Methods of Bacterial Concentration in Liquid Culture Conditions
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摘要 液体培养细菌后,分别测定培养液、培养液沉淀+无菌盐水的吸光度,结果存在明显差异。采用稀释平板法和显微计数法测定了培养液中细菌数量,并推导出嗜水气单胞菌活菌数与吸光度的关系为logI0/I=6.848×10-25·C。 Optical density ( OD ) of cultured solution, sediment of cultured solution, and sterile brine were tested respectively after bacteria were culture in liquid. The results were significantly different. The number of bacteria was counted with dilute plate method under microscope. Relationship between live bacteria of Aeromonas hydrophila and 019 was deduced as logI0/I = 6.848×10^-25·C.
作者 杨广
出处 《微生物学杂志》 CAS CSCD 2005年第4期71-72,共2页 Journal of Microbiology
关键词 细菌 浓度 吸光度 bacteria concentration optical density (OD)
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参考文献2

  • 1范秀容.微生物学实验[M].北京:高等教育出版社,1980..
  • 2微生物研究法讨论会.微生物学实验法[M].北京:科学出版社,1981.155-156.

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