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发酵条件对木霉菌株T23的菌丝生长及几丁质酶活性的影响 被引量:10

Influence of Fermentation Conditions on Hyphae Growth and Chitinase Activity of Trichoderma Strain of T23
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摘要 为使木霉生防制剂实现工业化生产,本试验对木霉菌株T23的最适菌丝生长和最佳产几丁质酶的发酵条件进行了研究。试验结果得出T23的最佳发酵条件以PDB为培养基,在250mL三角瓶中50mL装瓶量,接种量为6%,起始pH值为5,温度25℃离心机转速180r.min-1,培养4d,发酵液中菌丝产量和几丁质酶活分别达到10.64g.L-1和421.3U.mL-1。 The optimal hyphae growth conditions of strain of Trichodema Harianum T23 and its optimal fermentation conditions for chitinase-producing was performed in order to provide the scientific bases for industrial production of this biocontrol preparation. The results showed that the optimal conditions were as follows: 50mL medium of potato dextrose broth filled in 250mL flask with 6% inoculum of the culture, initial pH5, at 25℃, 180rpm incubation for 4d. The hyphae quantity and chitinase activity were 10.64g·L^-1 and 421.3U·mL^-1 respectively.
出处 《沈阳农业大学学报》 CAS CSCD 北大核心 2005年第3期332-335,共4页 Journal of Shenyang Agricultural University
基金 辽宁省博士启动基金项目(20021042)
关键词 木霉 几丁质酶 发酵 生物防治制剂 Trichodema spp. ehitinase fermentation biocontrol preparation
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