摘要
目的研究过量氟对大鼠肾细胞中游离钙( [Ca2+] i )水平的影响与氧化应激的关系。方法应用饮水加入氟化钠进行大鼠染毒实验,采用Fura鄄2/AM荧光指示剂测定慢性氟中毒大鼠肾细胞内[Ca2+] i浓度的变化,同时测定谷胱甘肽过氧化物酶(GSH鄄Px)活性、超氧化物歧化酶(SOD)活性和脂质过氧化终产物丙二醛(MDA)生成。结果过量氟可刺激肾细胞内[Ca2+] i浓度增高,高钙加氟组与高钙对照组相比差异有显著意义(P< 0.05),低钙加氟组高于低钙对照组(P< 0.001);SOD活力水平低钙加氟组低于低钙对照组,差异有显著意义(P< 0.05),MDA生成低钙加氟组高于低钙对照组(P< 0.05);GSH鄄Px活力变化不明显。结论(1)过量氟所致的大鼠细胞内[Ca2+] i超载和不同程度的氧化侵袭,可能在氟骨症病理过程中起重要作用;(2)投氟伴随低钙可加重细胞内[Ca2+] i超载和氧化侵袭,提示钙营养与氟中毒发病有着重要联系。
Objective To study the effect of intake of excessive fluoride on intracellular ionized calcium level and oxidative stress in renal of Wistar rats. Methods Sodium fluoride was feeded by drinking water. The Fura-2/AM was employed to measure the renal [Ca^2＋] i of Wistar rats; the GSH-Px, SOD and MDA were tested by biochemical methods. Results There was obviously increase in the fluoride exposed group than that in the control group （P〈0.05）; the same increase was observed between the group of low calcium ＋ fluoride and the low calcium group. The activity of SOD was lower in the group of low calcium ＋ fluoride than that of the low calcium group. Compared with the group of low calcium, the group of low calcium ＋ fluoride showed higher level MDA. Conclusion （1） The higher level of intracellular ionized calcium and oxidative stress are possibly significant in fluorosis pathological injury; （2） The level of excessive [Ca^2＋] i and oxidative stress in the fluoride exposed group are higher along with low calcium, which means calcium nutrition has close connection with fluorosis mechanism.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2004年第1期25-27,共3页
Chinese Jouranl of Endemiology
基金
国家自然科学基金重点项目(39730390)
关键词
大鼠
动物实验
肾细胞
内钙水平
氧化应激损伤
氟中毒
Fluorosis
Renal tissue
Intracellular ionized calcium
Nephrology oxidative stress
