摘要
【目的】探讨组织工程化人工骨应用于兔腰椎横突间融合的骨愈合生物学特点及其融合效果。【方法】45只新西兰大白兔均行单节段双侧L5／6横突间融合术，依植骨材料随机分为A(自体髂骨)、B(组织工程化人工骨)和C(单纯支架)3组，每组15只。术后2、6、12周分别处死动物3、3、9只，取出腰椎，通过大体观察、影像学、组织学和生物力学等方法分析融合状况。【结果】术后2周3组均未见骨性融合，12周大体标本观察A、B、C组融合率分别为66.67％(8／12)、83.67％(10／12)、0；生物力学测试A、B组融合者硬度和强度显著高于C组(P＜0.01)，A、B两组无明显差异(P＞0.05)；组织学示B组融合块中央区典型软骨细胞较少，纤细编织骨包围支架材料周围，此区新生骨以组织工程骨的直接成骨为主。【结论】组织工程化人工骨用于兔腰椎横突间融合，可达到自体髂骨的融合效果，未吸收支架材料不影响局部生物力学功能，中央区骨形成主要是直接成骨过程，边缘区包括膜内成骨、软骨成骨和直接成骨作用。
[Objective] To assess the bone healing biology of the tissue engineering bone as a kind of bone graft substitute and its fusion results in a rabbit model of posterolateral lumbar intertransverse process fusion. [Methods] Forty-five New Zealand white rabbits underwent single-level bilateral intertransverse process spine arthrodesis at L5/ 6. These rabbits were assigned to one of three groups according to the graft materials they received： autogenous iliac crest bone （group A, n=15）; tissue engineering bone （group B, n=15）; and hydroxyapafite/tricalcium phosphate carrier alone （group C, n=15）. Three, three, and nine animals were killed at 2, 6, and 12 weeks after surgery respectively and the spine fusion results were assessed by manual palpation, radiography, histology, and tensile biomechanical testing. [Results] There was no fusion can be seen in all groups at 2 weeks postoperatively. The fusion rate of group A, B, and C at 12th week were 66.67% （8/12）, 83.67% （10/12）, and 0, respectively. Tensile strength and stiffness of the solidly fused levels in group A and group B were statistically higher than that of group C （P〈 0.01）, but there was no difference between group A and group B （P 〉0.05）. Histological analysis showed that there were some typical chondrocytes in central area of fusion mass in group B. Carrier materials were surrounded by some thin woven bone. The de novo bone in this area was mainly come from the direct osteogenesis.[Conclusion]The tissue engineering bone graft is proved to be a suitable bone graft substitute in the lumbar intertransverse process spine fusion model in rabbit. Its fusion results were same as that of autogenous iliac bone. The residual carrier materials in fusion mass did not interfere with its biomechanical function. The healing biology process of this spinal fusion model with tissue engineering bone graft is characterized by its primary direct bone formation in central zone of fusion mass. Membranous bone formation, direct bone formation, and endochondral bone formation can be seen in marginal zone.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2005年第6期626-630,634,共6页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金(50272021) 国家自然科学基金(59872010)
关键词
兔
脊柱融合
植骨材料
组织工程化人工骨
rabbit
spine fusion
bone graft substitutes
tissue engineering bone
