摘要
本研究观察12例健康供者在使用10μg/(kg·day)rhGCSF动员前后白细胞总数变化。应用外周血涂片瑞氏染色对白细胞进行形态学分类,使用流式细胞术分析动员前后外周血单个核细胞中T细胞、B细胞、NK细胞和单核细胞比例的变化。结果发现,动员前1天外周血白细胞计数中位数为6.25(4.7-7.8)×109/L,其中淋巴细胞中位数为2.07(1.63-3.1)×109/L,单核细胞中位数为0.163(0.078-0.414)×109/L;动员第5天外周血白细胞计数中位数为37.47(24-72.57)×109/L,其中淋巴细胞中位数为3.22(1.46-5.31)×109/L,单核细胞中位数为1.2(0.706-3.627)×109/L。供者外周血白细胞的增加为动员前的6.26±2.14倍(P<0.01),其中淋巴细胞的增加为动员前的1.45±0.76倍(P<0.05),单核细胞数增加为动员前的7.48±4.41倍(P<0.01)。流式细胞术分析发现,动员前CD3+T淋巴细胞占外周血单个核细胞(PBMNC)比例的中位数为46.96%[(32.36-57.45)%],动员后为40.94%[(25.31-48.9)%];动员前CD4+/CD8+淋巴细胞比例为1.27±0.46,动员后为1.36±0.51;动员前CD4+CD8+T淋巴细胞占PBMNC比例的中位数为0.41%[(0.16-1.51)%],动员后为0.49%[(0.09-2.0)%];动员前CD16+CD56+NK细胞占PBMNC比例的中位数为13.98%[(4.08-25.08)%],动员后为16.65%[(12.06-33.05)%];动员前CD3+CD16+CD56+NK-T细胞占PBMNC比例的中位数为2.75%[(0.37-6.38)%],动员后为3.13%[(0.46-5.95)%];动员前CD20+B淋巴细胞占PBMNC比例的中位数为9.28%[(5.97-16.33)%],动员后为9.94%[(7.36-20.41)%];动员前CD14+单核细胞占PBMNC比例的中位数为12.48%[(3.54-19.35)%],动员后为29.52%[(16.51-36.76)%]。动员后CD14+单核细胞在PBMNC中的比例比动员前增加2.87±1.51倍(P<0.05);动员前后T淋巴细胞、NK细胞、NK-T细胞、B淋巴细胞在PBMNC中的比例以及动员前后CD4+/CD8+淋巴细胞比均无显著变化(P>0.10)。结论:rhGCSF动员引起的单核细胞增加可能在异基因外周血造血干/祖细胞移植的相关事件中发挥着重要作用。
To investigate the changes of donorg peripheral blood immunocytes after mobilization with medium - dose recombinant human granulocyte -colony stimulating factor（ rhG-CSF）, the amounts of immunocytes in peripheral blood cells and the immunocyte components of donor peripheral blood mononuclear cells（PBMNC） in 12 healthy donors were detected by flow cytometry before and after mobilization with rhG-CSF 10 μg/（ kg·day）. The results showed that the median amounts of peripheral blood leukocytes before mobilization was 6.25 （4.7 -7.8 ）×10^9/L, for lymphocytes it was 2.07 （1.63 -3.10）×10^9/L, and for monocytes it was 0. 163 （0,078 -0.414）×10^9/L. In the fifth day after mobilization, the median amounts of peripheral blood leukocytes was 37.47 （24 -72.57 ）×10^9/L, and for lymphocytes it was 3.22 （ 1. 46 -5.31）×10^9/L, and for monocytes, it was 1.2 （0. 706 -3. 627 ）×10^9/L. The average amount of leukocytes after mobilization was 6.26 ±2.14 multiple of that before mobilization（ P 〈0.01） ,and the median amounts of lymphocytes after mobilization was 1.45 ± 0.76 multiple of that before mobilization （ P 〈 0.05 ）, and the amount of monocytes after mobilization was 7.48 ± 4.41 multiple of that before mobilization （ P 〈 0.01 ）. The median percentage of CD3^＋ T lymphocytes before mobilization was 46.96% [ （ 32.36 - 57.45 ） % ] , but 40.94% [ （ 25.31 - 48.9 ） % ] after mobilization. The ratio of CD4^＋/CD8^＋ before mobilization was 1.27 ± 0.46, while 1.36 ± 0.51 after mobilization. The median percentage of CD4^＋CD8^＋T lymphocytes was 0.41% [ （0. 16 - 1. 51 ）% ] , and 0. 49% [ （0.09 - 2.0） % ] after mobilization. The median percentage of CD16 ^＋ CD56 ^＋NK cells was 13.98% [ （ 4.08 - 25.08 ） % ] vevsus 16.65% [ （ 12.06 - 33.05 ） % ] after mobilization. The median percentage of CD3^＋ CD16^＋ CD56^＋ NK-T cells was 2.75 % [ （0.37 - 6.38 ） % ] , but 3.13 % [（ 0.46 - 5.95 ） % ] after mobilization. The median percentage of CD20^＋ B cells was 9.28% [（ 5.97 - 16.33 ） % ] , while 9.94% [ （7.36 - 20.41 ） % ] after mobilization. The median percentage of CD14^＋ monocytes was 12.48% [ （ 3.54 - 19.35 ） % ] vevsus 29.52% [ （ 16.51 - 36.76 ） % ] after mobilization. The percentage of CD3^＋ T lymphocytes, CD4^＋ CD8^＋ T lymphocytes, NK cells, NK-T cells and B lymphocytes in PBMNC did not change markedly before and after mobilization with middle-dose rhG-CSF. The ratio of CD4^＋/CD8^＋ did not change significantly （P 〉 0. 10）. The percentages of CD14^＋ monocytes in PBMNC after mobiliztion increased up to 2.87 ± 1.51 higher than that before mobilization （P 〈0.05）. It is concluded that the changes of the CD14^＋ monocytes aftermobilization with rhG-CSF may be involved in graft rejection and graft versus host disease after allo-PBSCT.
出处
《中国实验血液学杂志》
CAS
CSCD
2005年第5期852-856,共5页
Journal of Experimental Hematology
基金
解放军总医院十五科研计划基金重点资助项目
编号01YZ05
关键词
重组人粒细胞集落刺激因子
动员
免疫细胞
外周血
recombinant human granulocyte-colony stimulating factor
mobilization
immunocyte
peripheral blood
