期刊文献+

血液中ATP含量随保存温度和保存时间变化的规律性研究及数学建模 被引量:4

Trendency and Mathematical Model of Relationship of Blood ATP Content with Temperature and Time of Preservation
下载PDF
导出
摘要 为了研究血液中ATP含量随保存温度和保存时间变化的规律性并建立数学模型,以ATP含量作为血液质量的评判指标,对4-32℃区间不同保存温度条件下血液的质量变化进行了系统的研究,得出系列经验数据。结果表明:假设ATP的浓度y=f(d,t,s)是时间d、温度t和起始浓度s的连续函数,利用数理统计中的线性回归理论对模型加以拟合,得到了ATP浓度随保存温度和保存时间变化的一般数学物理方程,根据方程推算在4、6、8、10、12、14、16、18、20、22、24、26、28、30和32℃温度下,以CPDA1保存的全血分别可以有效保存35、35、29、22、18、18、13、8、7、6、6、5、4、4和3天。结论:本研究首次系统揭示了保存温度变化时血液质量变化的一般性规律,为准确判断变温血质量、指导临床合理用血提供科学参考。 To investigate the relationship of blood ATP content with temperature and time of preservation and to establish its mathematical model, adenosine triphosphate (ATP) concentration was applied as the index of the quality of erythrocytes; systematical study on variation of blood preserved in a series of different temperatures from 4℃ to 32℃ was perfomed, and a series of experimental data were obtained. The results showed that when the ATP concentration y = f (d, t, s) in preserved blood was given as the continuous function of the time (d), the temperature (t) and the initiate ATP concentration (s), the model was fitted with the theory of linearity regression in symbolic statistics, and the general mathematical physical equation of the variation of preserved blood quality was deduced. According to the equation, the whole blood in CPDA-1 solution could be efficiently stored for 35, 35, 29, 22, 18, 18, 13, 8, 7, 6, 6, 5, 4, 4 and3 days in 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30 and 32℃, respectively. In conclusion, the general trendency of the variation of preserved blood quality according to the temperature and the time was systematically disclosed for the first time, which would be propitious to estimate the blood quality in various temperatures and to instruct clinical blood transfusion.
出处 《中国实验血液学杂志》 CAS CSCD 2005年第5期896-900,共5页 Journal of Experimental Hematology
基金 全军医学科研"十五"计划课题资助项目 编号01L040
关键词 血液保存 ATP 线性回归 数学模型 Blood preservation Adenosine triphosphate Linearity regression Mathematical model
  • 相关文献

参考文献11

  • 1Knight JA, Searles DA, Blaylock RC. Lipid peroxidation compared in stored whole blood with various nutrient-anticoagulant solutions.Ann Clin Lab Sci, 1993; 23:178 - 183.
  • 2Turkes S, Korkmaz O, Korkmaz M. Time course of the age-related alterations in stored blood. Biophys Chem, 2003; 105:143 -150.
  • 3Ruddell JP, Lippert LE, Babcock JG, et al. Effect of 24-hour storage at 25 degrees C on the in vitro storage characteristics of CPDA-1 packed red cells. Transfusion, 1998; 38:424 -428.
  • 4中国医学科学院输血研究所血液保存组.保存红细胞质量体外检查法的初步研究[J].中国科学,1977,7:369-375.
  • 5Dern R J, Brewer GJ, Wiorkowski JJ. Studies on the preservation of human blood. Ⅱ. The relationship of erythrocyte adenosine triphosphate levels and other in vitro measures to red cell storageability. J Lab Clin Med, 1967; 69:968 -978.
  • 6Carmen RA, Sohmer PR, Leng BS, et al. Five-week red cell storage with preservation of 2,3 DPG. Transfusion, 1988; 28:157 -161.
  • 7Pittiglio DH. Modern blood banking and transfusion practices. Philadelphia: Davis FA Company. 1983: 3 -9.
  • 8Hess JR, Greenwalt TG. Storage of red blood cells: new approaches.Transfus Med Rev, 2002; 16: 283 - 295.
  • 9Saint-Blancard J, Allary M, Fabre G, et al. Properties of red blood cell concentrates stored in PAGGS-sorbitol. Ann Pharm Fr, 1995;53: 220 - 229.
  • 10Hess JR, Lippert LE, Derse-Anthony CP, et al. The effect of phosphate, pH, and AS volume on RBCs stored in saline-adenine-glucose-mannitol solutions. Transfusion, 2000; 40:1000 - 1006.

共引文献1

同被引文献56

  • 1滕本秀,王竹筠.库存血红细胞流变特性的变化[J].临床输血与检验,2000,2(4):11-12. 被引量:9
  • 2邱艳,杨海平,查祎,李立立,李素波,汪德海,张志欣,章扬培.人血浆对红细胞保存的影响[J].中国实验血液学杂志,2005,13(4):692-697. 被引量:10
  • 3樊凤艳,周虹.红细胞4℃保存研究进展[J].中国输血杂志,2006,19(2):155-157. 被引量:9
  • 4骆群,韩玮,刘景汉.海藻糖在血液保存中的作用[J].临床输血与检验,2006,8(3):167-170. 被引量:5
  • 5Pittiglio DH. Modem blood banking and transfusion practices[M]. Philadelphia: Davis FA Company, 1983:3-9
  • 6Dumaswala U J, Zhuo L, Jacobsen DW et al. Protein and lipid oxidation of banked human erythrocytes : role of glutathione[J]. Free Radic Biol Med, 1999,27 (9-10) : 1 041- 1 049
  • 7Hogman CF. Liquid - stored red blood cells for transfusion. A status report [J]. Vox Sang, 1999,76 ( 2 ) :67-77
  • 8Korgun DK, Bilmen S, Yesilkaya A. Alterations in the erythrocyte antioxidant system of blood stored in blood bags [J]. ResCommun Mol Pathol Pharmacol,2001,109 (5 - 6) :357-363
  • 9Dumaswala U J, Wilson M J, Wu YL, et al. Glutathione loading prevents free radical injury in red blood cells aftel storage[J]. Free Radic Res,2000,33(5):517-529
  • 10Hess JR, Lippert LE, Derse - Anthony CP, et al. The effects of phosphate, pH, and AS volume on RBCs stored in saline - adenine - glucose - mannitol solutions [ J ]. Transfusion, 2000, 40 (8) :1 000-1 006

引证文献4

二级引证文献27

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部