摘要
以酚醛树脂作载体,研究了糖化型淀粉酶固定化条件及固定化糖化酶的性质.结果表明,在 pH=3,T=35℃,t=4h 条件下所得固定化酶活力最高,与游离酶相比,酶活力回收可达82%.糖化酶经固定后催化反应最适 pH=4.2,最适温度为48℃,K_m=28.6mg/mL,装柱连续水解淀粉240h 酶活力保留87%.
The phenolic resin was used as carrier to immobilize the glucoamylase.The factors which effect the process of immobilizing were studied using orthogonal test.The best condi- tion of immobilizing is:pH=3,T=35℃,t=4 h and 82% activity of glucoamylase was con- served.The optimum conditions of immobilized glucoamylase action on the substance (starch)are:pH=4.2,T=48℃.K_■=28.6 mg/mL.Continous hydrolysis amylum 240 h.so 87% activity of glucoamylase was conserved.The result shows the immobilized glucoamylase stability on time is high.
出处
《郑州轻工业学院学报》
1996年第1期50-54,共5页
Journal of Zhengzhou Institute of Light Industry（Natural Science）
关键词
葡萄糖淀粉酶
固相酶
酚醛树脂
载体
enzyme
glucoamylase
immobilized enzyme
saccharifying agents
resin
