摘要
狂犬病毒CVS株免疫BALB／c小鼠后，获得6株分泌单克隆抗体的杂交瘤细胞株，经检测，其中4株分泌抗狂犬病毒核蛋白单抗．2株分泌抗糖蛋白单抗．将这些单抗腹水制成狂犬病毒ELISA夹心法酶标诊断试剂，试验结果表明，对固定每株及街毒有较好的特异性．对正常鼠脑及组织培养物无非特异性反应，可检出毒力低至330LD50的CVS林狂犬病毒，有较好的敏感性。
Six hybridoma cell strains secreting McAbs were obtained by immunizing BALB/c mice with CVS strain of rabies virus. We found that 4 of the 6 strains secreted McAbs to rabies virus glycoprotein, and the other 2 of them secreted McAbs to rabies virus nucleoprotein. The dignostic reagent for rabies virus by sandwich ELISA was prepared with the ascites containing these McAbs and showed good specificity to both fixed and street virus strains, but no non-specific reaction to normal mice brain or tissue ctllture was found. The CVS strain with a low titer of 330 LD50 was detectable and this indicated its good sensitivity.
出处
《中国生物制品学杂志》
CAS
CSCD
1996年第1期23-24,共2页
Chinese Journal of Biologicals
