摘要
采用柱层析法提纯狂犬病毒的研究顾鸣，邵益斌，顾勤，曾蓉芳（卫生部上海生物制品研究所，上海２０００５１）关键词狂犬病毒，柱层析法迄今，分离提纯包膜病毒的方法多采用密度梯度离心法、亲和层析法和等电聚焦法等，这些方法有一定的优点，但用在处理量很大的病毒疫苗...
he active Rabies virus suspension was cultured in microcarrier culture of five litres bioreac-tor.After virus suspension was havested from microcarrier culture,the prdcess comprised clarifi-cation by centrifugation and filtration,concentration by ultrafiltration,purification by gel filtra-tion.The virus was inactivated with 1:4000β-propiolactone at 4℃for 24 hours.The results indicated that infective titer of the rabies virus was not declinged clearly,thatresidual calf serum protein,residual DNA content and antigenic values(NIH test)in all purifiedvirus samples were conformed to the WHO standards.The research described in this paper indicated that isolating,purifying method of active ra-bies virus was effective, It provided some basic information which could be useful in further forthe largescale production of inactivated rabies vaccines.
出处
《中国病毒学》
CSCD
1996年第1期81-83,共3页
Virologica Sinica
关键词
狂犬病毒
柱层析法
疫苗
Rabies Virus,Column chromatography
