摘要
目的探讨三价铬改善糖代谢的作用机制。方法以血铬正常的健康人作对照,将63例血铬降低的T2DM患者的红细胞悬液与不同浓度的三氯化铬进行体外孵育,测定孵育前、后碘标INS与红细胞INS受体结合物的放射性,并进行Scatchard分析。结果T2DM组较对照组血铬显著降低(P<0.01);碘标INS与受体的最大特异性结合率及r1、r2均降低(P<0.01),且空腹INS水平与受体的结合位点之间呈显著的负相关(r=-0.91,P<0.05)。加入三氯化铬后,与“0”浓度组比较,“100”浓度组仅r1增加(P<0.01),而“200”及“400”浓度组的最大特异性结合率及r1、r2均增加,k1及k2降低,均有统计学差异。结论三价铬可能通过增加受体的敏感性而增加INS与受体的结合率,从而改善IR调节糖代谢。
[Objective] To explore the action mechanism of trivalent chromium improving glycometabohsm. [Methods] With normal serum chromium objects as controls, erythrocytic suspensions from 63 cases with type 2 diabetes and lower serum chromium were incubated in vitro with different concentrations of chromium trichloride. Radioactivity of iodine labeled insulin and erythrocytic insulin receptor conjugate before and after s/he incubation was measured and Scatchard analysis was done. [Results] Compared with controls, serum chromium, maximum specific combining rate between iodine labeled insulin and receptor, and high and low affinity receptor combining sites （r1 and r2） were reduced in type 2 diabetes group （P 〈0.01）. In type 2 diabetes group the correlation between fasting insulin level and receptor combining sites presented obviously negative correlation （r =-0.91, P 〈0.05）. After adding chromium trichloride, compared with 0 concentration group only r1 was increased in 100 concentration group （P 〈 0.01）. In 200 and 400 concentration groups maximum specific combining rates, r1 and r2 were increased, but two receptor affinity constants （k1 and k2） were reduced （P 〈0.05）. [Conclusion] Trivalent chromium may increase the combining rate between insulin and receptor through enhancing receptor＇ s sensitivity so as to improve insulin resistance and to regulate glycometabelism.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第20期3089-3092,共4页
China Journal of Modern Medicine
关键词
铬
2型糖尿病
红细胞胰岛素受体
胰岛素抵抗
chromium
type 2 diabetes mellitus
insulin resistance erythrocyte
insulin receptor
