摘要
目的探讨中国北方汉族人群基质金属蛋白酶1(MMP1)基因多态性与慢性阻塞性肺疾病(COPD)易感性的关系。方法应用聚合酶链反应限制性片段长度多态性法(PCRRELP)检测MMP1基因型在59例中国北方汉族人COPD患者和109例健康人中频率的分布,用回顾性病例对照实验设计分析人群对COPD易感性的相对危险度及相应可信区间。结果COPD患者MMP1基因的突变型(2G/2G)、杂合型(2G/1G)、野生型(1G/1G)频率分布分别为54.2%(32/59)、37.3%(22/59)和8.5%(5/59),健康人为71.5%(78/109)、20.2%(22/109)和8.3%(9/109)。以野生型(1G/1G)与杂合型(2G/1G)为参考组,MMP1基因突变型(2G/2G))与参考组相比,OR=0.467,95%CI=0.237～0.924,差异有统计学意义(P<0.05)。结论MMP1基因突变型(2G/2G)降低中国北方汉族人群对COPD的遗传易感性。
Objective To study the relationship between the matrix metalloproteinase-1 （ MMP-1 ） gene polymorphism and genetic susceptibility to chronic obstructive pulmonary disease（COPD） in Han nationality of northern China. Methods The genotypes of MMP-1 gene in 59 patients with COPD and 109 healthy controls were detected by polymerase chain reaction（PCR） based restriction fragment length polymorphism（RFLP） technique. Retrospective case-contrnl study was performed to analyze the odds ratio （ OR ） which was representative of the relative risk rate of COPD susceptibility. Results The frequency of MMP-1 2G/2G,2G /1G, 1G/1G genotype were 54.2% （32/59）,37. 3% （22/59）, 8.5% （5/59） in COPD group, respectively, and 71.5% （78/109） ,20.2% （22/109）,8.3% （9/109） in control group,respectlvely.Taklng 1G/1G and 2G/1G group as the reference, the 2G/2G group showed a reduced risk rate（ OR = 0.467,95 % CI= 0. 237 - 0.924） with a statistically significant difference（ P 〈 0.05）.Conclusion MMP-1 （2G/2G） genotype reduces the suseeptibility to COPD in Han nationality of northern China.
出处
《中国呼吸与危重监护杂志》
CAS
2005年第5期342-344,共3页
Chinese Journal of Respiratory and Critical Care Medicine
