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分子生物学在石油微生物多样性研究中的应用 被引量：10

Application of Molecular Biotechniques in Research on Petroleum Microbial Diversity
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摘要 该文较系统地概述了目前以16S rRNA为基础的核酸探针检测技术、利用引物的PCR技术、DNA序列分析技术和电泳分离及显示技术在油藏微生物群落多样性和石油环境污染研究中的研究进展,并探讨了未来分子生物学技术在石油微生物研究中的发展方向。
作者 王大威 杨振宇 石梅 刘永建
机构地区 大庆石油学院 大庆勘探开发研究院
出处 《生物技术》 CAS CSCD 2005年第5期86-89,共4页 Biotechnology
关键词 16S rRNA石油微生物多样性 生物修复
分类号 Q145 [生物学—生态学] Q78 [生物学—分子生物学]
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参考文献18

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  • 2Gerrit Voodrdouw, Stephen M Armstrong, Monika F Reimer, et al. Characterization of 16S rRNA genes from oil field microbial communities indicates the presence of a variety of sulfate - reducing, fermentative, and sulfide - oxidizing bacteria[J] .Appl. Envir. Microbiol, 1996,62(5): 1623 - 1629.
  • 3Yoh Takahata, Miyuki Nishijima, Toshihiro Hoaki, et al. Distribution and physiological characteristics of hyperthemophiles in the Kubiki oil reservoir in Niigata, Japan[J]. Appl. Envir. Microbiol,2000,66( 1 ) :73 - 79.
  • 4Kazuya Watanabe, Kanako Watanabe, Yumiko Kodama, et al. Molecular Characterization of Bacterial Populations in Petroleum- Contaminated Groundwater Discharged from Underground Crude Oil Storage Cavities[J]. Appl. Envir.Microbiol,2000,66(11) :4803 -4809.
  • 5Yumiko Kodama and Kazuya Watanabe. Isolation and characterization of a Sulfur- Oxidizing chemolithotroph growing on crude oil under Anaerobic conditions[J]. Appl. Envir. Microbiol,2003,69(1):107-112.
  • 6Sharon A. Churchill, Jennifer P Harper, and Perry F Churchill, et al.Isolation and characterization of a Mycobacterium species capable of degrading three - and four- ring aromatic and aliphatic hydrocarbons[J] .Appl. Envir. Microbiol, 1999,65(2) :549 - 552.
  • 7Elizaveta A Bonch - Osmolovskaya, Margarita L Mireshnichenko, Alexander V Lebedinsky, et al. Radioisotopic, culture- based, and oligonucleotide microchip analyses of thermophilic microbial communities in a continental high -temperature petroleum reservoir[ J]. Appl. Envir. Microbiol, 2003,69 (10):6143 - 6151.
  • 8Kazuya Watanabe, Yumiko Kodama, Natsuko Hamamura, et al.Diversity,abundance, and activity of archaeal populations in oil - contaminated groundwater accumulated at the bottom of an underground crude oil storage cavity[J].Appl. Envir. Microbiol,2002,68(8) :3899 - 3907.
  • 9Wilfred F M Roling, Michael G Milner, D Martin Jones, et al. Bacterial community dynamics and hydrocarbon degradation during a field - acale evaluation of bioremediation on a mudflat beach contaminated with buried oil[J].Appl. Envirom. Microbial, 2004,70(7):2603 - 2613.
  • 10Pui- Yi Cheung, Brian K Kinkle. Mycobacterium diversity and pyrene mineralization in petroleum contaminated soils[J] .Appl.Environ.Microbiol,2001,67(5) :2222 - 2229.

二级参考文献28

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生物技术
2005年 第5期

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