摘要
目的研究紫球藻胞外多糖的分离及体外抗乙肝病毒活性。方法紫球藻培养液通过离心、浓缩、透析、醇析、脱蛋白、冷冻干燥等步骤从中分离出胞外多糖;采用ELISA和MTT法测定紫球藻胞外多糖对2215细胞分泌e抗原(HBeAg)的影响。结果元素分析表明,胞外多糖含N:0.82%、C:32.91%、H:6.19%;氨基酸分析表明,含有17种氨基酸,含量为2.49%。红外光谱和紫外光谱分析表明,所分离的胞外多糖具有多糖的特征吸收峰,糖环为吡喃环,含有硫酸酯基团。该胞外多糖对2215细胞分泌的HBeAg有不同程度的抑制作用,其治疗指数(TI)大于2。结论紫球藻胞外多糖在体外具有抗乙肝病毒作用。
Objective To investigate the separation of extracellular polysaccharide from Porphyridiurn cruentum and its anti-HBV activity in vitro. Methods The extracellular polysaccharide（EP） of Porphyridium cruentum was extracted and isolated from the culture medium. HBeAg was assayed by ELISA and MTT methods. Results Element analysis showed that the extracellular polysaccharide contained N 0.82%,C 32.91% ,H 6.19% and amino acid 2. 49% . The IR and UV spectrum of EP determination showed typical absorption curve of polysaccharide, pyranglycoside linkage, sulfate ester. EP had a significant inhibition to HBeAg excretion with low toxicity on the cells and a better therapeutic index（TI）. Conclusion The results suggested that EP has inhibitory effect on the hepatitis B virus in vitro.
出处
《中国海洋药物》
CAS
CSCD
2005年第5期18-21,共4页
Chinese Journal of Marine Drugs
基金
福建省发展计划委员会(闽计投资[2003]203)
福建省教育厅资助项目(JA03042)
关键词
紫球藻
胞外多糖
分离
乙型肝炎病毒
Porphyridium cruentum
extracellular polysaccharide
separation
hepatitis B virus（HBV）
