摘要
目的研究单酶和复合酶水解红非鲫鱼皮胶原蛋白制备具有抑制血管紧张素转换酶(ACE)生物活性的寡聚肽的工艺条件。方法根据6种单酶的水解产物对ACE抑制作用的测定结果,确定了两种酶即菠萝蛋白酶和alcalase 2.4L碱性蛋白酶组成复合水解酶。采用正交试验确定了这两种酶单独水解鱼皮胶原蛋白的最适酶解条件。结果与结论菠萝蛋白酶和alcalase 2.4L蛋白酶的最适酶解温度,pH,酶/底物,时间,底物浓度分别为45℃,pH4.5,4000U.g-1,4h,6%和55℃,pH7.5,6000U.g-1,2h,4%。在此基础上,进行复合酶水解实验,结果表明先采用菠萝蛋白酶水解,再用alcalase 2.4L蛋白酶水解,效果更佳。采用该双酶复合水解工艺,所得水解产物的水解度为30.43%,体外对ACE的抑制率达68.6%。
Objective To study the single and combined-enzymic hydrolysis conditions of Oreochromis niloticus skin collagen. Methods Based on the inhibitory activities of six kinds of single enzymic hydrolysates to angiotensin converting enzyme （ACE）, bromelain and alcalase 2.4L were selected as combined-enzyme. The optimal hydrolytic conditions of the skin collagen with the two enzymes were determined by orthogonal test LaG （4s）. Results and Conclusion The results showed that the optimal hydrolytic conditions of bromelain and alcalase 2.4L were temperature 45℃, pH4.5, E/S 4000U · g^-1 time 4h,concentration of substrate 6%; and temperature 55℃, pHT. 5, E/S 6000U · g^-1 , time 2h, concentration of substrate 4%, respectively. Furthermore, hydrolytic effect would be better when using bromelain in advance, followed by alcalase 2.4L. The hydrolytic degree of the hydrolysates prepared under the provided technology was 30.43 %, the ACE inhibitory rate would be as high as 68.650% in vitro.
出处
《中国海洋药物》
CAS
CSCD
2005年第5期24-29,共6页
Chinese Journal of Marine Drugs
基金
青岛市重点攻关课题(03-1-HH-12)
广东省科技计划项目(2004B20401006)
关键词
红非鲫鱼皮
胶原蛋白
复合酶解
水解度
血管紧张素转换酶
Oreochromis niloticus skin
collagen
combined-enzymic hydrolysis
degree of hy-drolysis
angiotensin converting enzyme （ACE）
