摘要
目的探讨生物可降解材料聚乳酸-聚乙醇酸-聚乙二醇(PLGA-PEG)与血管平滑肌的细胞相容性,为可降解血管支架材料的研究提供依据。方法采用体外培养的兔血管平滑肌细胞种植在PLGA-PEG膜片上,用相差显微镜观察细胞的生长情况,每天计数,绘制细胞生长曲线,用MTT法测定细胞增殖指数,流式细胞仪检测细胞周期分布。结果兔血管平滑肌细胞在PLGA-PEG膜片上生长良好,与对照组差异无显著意义;MTT法检测细胞增殖指数,与对照组差异无显著意义;流式细胞仪检测细胞周期显示对细胞增殖活性无明显影响。结论PLGA-PEG与兔血管平滑肌细胞具有良好的细胞相容性,可以用于可降解血管支架的制备。
Objective To explore the cytocompafibility of biodegradable polylactic acid-polyglycolic acid-polyethylene（PLGA-PEG） with vascular smooth muscle cells and provide a basis for the development of biodegradable material for vessel scaffold. Methods Inoculate the rabbit vascular smooth muscle cells cultured in vitro onto PLGA-PEG membrane and observe their growth by phase contrast microscopy. Count the cells daily and plot a cell growth curve. Determine the proliferation of cells by MTF method and the distribution of cell cycle by flow cytometer.Results Rabbit vascular smooth muscle cells grew well in PLGA-PEG membrane and showed no significant difference with control.The determination result of cell cycle showed that the proliferation activity of cells were not influenced significantly. Conclusion PLGA-PEG showed good cytocompatibility with rabbit vascular smooth muscle cells. The material might be used for the production of biodegradable vessel scaffold.
出处
《中国生物制品学杂志》
CAS
CSCD
2005年第6期477-479,共3页
Chinese Journal of Biologicals
