摘要
目的探讨高同型半胱氨酸血症是否引发兔主动脉的血管炎症反应。方法20只雄性新西兰兔随机分成对照组(正常饮食)和蛋氨酸组(高蛋氨酸饮食),每组各10只,12周后测定血浆同型半胱氨酸,观察主动脉病理变化,酶联免疫吸附法检测白细胞介素8蛋白水平,免疫组织化学检测血管核因子κB的表达和活化。结果高蛋氨酸饮食显著增加血浆同型半胱氨酸水平,蛋氨酸组主动脉切片发现内皮细胞脱落,局部内膜增厚;白细胞介素8分泌增加;主动脉内皮细胞核因子κB表达增强。结论高同型半胱氨酸血症可以导致血管炎症反应,而炎症反应是高同型半胱氨酸血症加速动脉粥样硬化潜在的分子机制之一。
Aim To investigate whether hyperhomocysteinemia could induce vascular inflammstory response in aorta of rabbit. Methods Twenty New Zealand white rabbits were ramdomly divided into control group and methionine group. After twelve weeks, the level of homocysteine （Hey） were measured, aortic lesion were obsersved, Interleukin-8 （IL-8） were determined by enzyme-linked immunosorbent assay （ELISA）, the expression and activation of nuclear factor-κB （NF-κB were studied by immunohistochemistry. Results Hyperhomocysteinemia was induced after high methionine diet, aortic endotheliocyte came off and local infima thickened. The production of IL-8 and activation of NF-κB were increased by hyperhomocysteinemia. Conclusions Hyperhomocysteinemia can induce vascular inflammatory response. Inflammatory response is one of potential cellular mechanisms by which hyperhomocysteinemia accelerates atherosclerosis.
出处
《中国动脉硬化杂志》
CAS
CSCD
2005年第4期464-466,共3页
Chinese Journal of Arteriosclerosis
