摘要
目的寻找Battenin相互作用蛋白的筛选方法,并对筛选出的Battenin相互作用蛋白进行初步鉴定。方法将编码Battenin相互作用蛋白肽段的cDNA序列与plexA质粒的DNA结合结构域基因重组作为诱饵,应用MATCHMAKERLexA酵母双杂交系统从人胎脑cDNA文库中筛选与Battenin相互作用蛋白再发生相互作用的蛋白质的cDNA,并进行生物信息学分析。结果从胎脑cDNA文库中筛选得到7个阳性克隆,其中一个基因与人TEGT基因有99%的同源性,TEGT编码蛋白为Bax抑制子1。结论Battenin相互作用蛋白与Bax抑制子1或类似物相互作用,Bax抑制子1具有反式调控因子特性,二者都可能参与基因表达调控。
Aim To investigate the physiological role of Battenin interactive protein （BIP） and screen the proteins interacting with BIP in human fetal cDNA hbrary. Methods The cDNA coding BIP and binding domain of plexA were recombined as a “bait”, proteins interacting with BIP were screened from human fetal cDNA hbrary by yeast two-hybrid system. Results Seven positive clones were isolated through plexA-HERPUD1 screening the human fetal brain cDNA library by yeast bybridtechnique, One of them has 99 % homology with the TEGE gene of human, whichcodes Baxinhibitor-1 （BI-1）（testisenhanced gone transcript）. Bioinformatic analysis shows that： Bax inhibitor-1 which interacts with BCL-2 and BCL-X is a gene transcript enhanced factor and a regulatory factor of apoptosis and probably associated with gene expression regulation for cell＇ s growth and development. The gone that codes Bax inhibitor-1 is TEGT located in chromosome 12q12-q13, containing 8 extrons and 7 introns. Condusions The interactive proteins of BIP were screened through yeast two-bybrid system successfully, and one of the proteins is Bax inhibitor-1. Bax inhibitor-1 is a gene transcript enhanced factor and a regulatory factor of apoptosis and probably associated with gene expression regulation for cell＇ s growth and development.
出处
《中国动脉硬化杂志》
CAS
CSCD
2005年第3期287-291,共5页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金资助项目(30370795)
