摘要
从人组织型纤溶酶原激活剂(t～PA)cDNA 克隆 pHS-1获取 t-PAcDNA 片段,与质粒pUC_8构建了 t-PAcDNA 亚克隆。从该克隆中获得分离 t-PA 基因的探针.
We constructed a t-PAcDNA subclone(pHS-2) that contained a t-PAcDNA fragment from t-PAcDNA clone (pHS-1) ligated into plasmid pUC_8 at PstI site.The subclone was used as a probe for isolating of t-PA gene
出处
《遵义医学院学报》
1989年第1期25-29,共5页
Journal of Zunyi Medical University
关键词
克隆
亚克隆
DNA重组
质粒
clone
Subclone
plasmid
DNA-recombination
restriction endoenzyme
probe
