摘要
目的探讨HPV16感染与端粒酶hTERT表达在人宫颈鳞状细胞癌及其癌前病变中的关系。方法应用组织芯片技术结合原位杂交技术和免疫组织化学方法研究正常宫颈组织、宫颈上皮内肿瘤组织和宫颈鳞癌组织中HPV16感染以及hTERT表达的情况。结果CINⅡ级、CINⅢ级、浸润性鳞癌组织中HPV16杂交信号阳性率显著高于正常宫颈组织(P<0.05),浸润癌HPV16阳性率也显著高于CIN(P<0.05);hTERT在CINⅡ级、CINⅢ级、浸润性鳞癌组织中的表达都显著高于正常宫颈组织(P<0.05),浸润癌也显著高于CIN(P<0.05);HPV16感染与hTERT表达之间呈正相关(P<0.05,r=0.339)。结论宫颈鳞癌的形成与HPV16的感染、hTERT过度表达有重要关系。宫颈鳞癌及其癌前病变组织中HPV16感染与hTERT表达之间呈正相关,两者联合检测配合细胞学检查可能利于提高宫颈癌及其癌前病变的诊断率。组织芯片技术是高效的研究基因及其表达产物的技术平台。
Objective To investigate the relationship between HPV16 infection and the expression of telomerase hTERT in cervical intraepithelial neoplasia（CIN） and squamous cell carcinomas（SCC） of the cervix uteri. Method Tissue microarray combined with in situ hybridzafion（ISH） and immunohistochemical staining （EliVision^TM plus method） was used to detect the expression of HPV16 RNA and hTERT in all 130 cases of normal cervical tissue and CIN and SCC of the cervix uteri .Results The positive rate of HPV16 hibrid signals in CIN Ⅱ, CIN Ⅲ and SCC were all significantly higher than in normal cervical tissue（ P 〈 0.05）, and it was also higher in SCC than in CIN（P 〈 0.05）.The rate of positive expression of hTERT protein in SCC/CIN B/CIN Ⅱ were all significantly higher than in normal cervical tissue（ P 〈 0.05） and was also significantly higher in SCC than in CIN cases （P 〈 0.05）. The HPV16 infection and the positive expression of hTERT showed a positive correlation in the progress from CIN to SCC（ P 〈 0.05, r = 0. 339）. Conculusion There was a positive correlation between HPV16 infection and the expression of hTERT in process from CIN to SCC. Maybe it can revealed the detailed mechanism which HPV16 participate in malignant transformation. Also they can combined as cellular and viral biomarkers to help identify cervical carcinoma and cervical precursor lensions with a risk of malignant progression. Tissue microarray is an efficient technique to study genes and its expression product.
出处
《中国肿瘤临床与康复》
2005年第5期388-391,共4页
Chinese Journal of Clinical Oncology and Rehabilitation
