摘要
目的:了解急性坏死性胰腺炎(ANP)时大鼠回肠组织中P物质的表达及其与肠黏膜通透性的关系。方法:健康成年SD大鼠随机分为对照组40只和ANP组40只,对照组开腹后只翻动胰腺,ANP组大鼠胰胆管恒速逆行注射5%牛黄胆酸钠制成ANP大鼠模型,分别于制模成功后8、12、16和20 h处死大鼠采取标本。用同位素法测定肠黏膜通透性,免疫组织化学方法检测P物质在回肠组织中的表达水平和组织学定位。结果:ANP组大鼠回肠组织中P物质的表达以及肠黏膜通透性(99mTc-DT-PA排泄率)较对照组明显增加(P<0.05)。P物质在回肠组织中的表达与肠黏膜通透性呈明显正相关(r=0.66,P<0.01)。结论:ANP时大鼠回肠组织中P物质表达升高,且随时间延长而增加,其表达与大鼠肠黏膜通透性呈正相关。
Objective To investigate the expression of substance P in rats＇ intestinal tissue and study the relation between substance P and mucosa permeability in acute necrotizing pancreatitis （ANP）. Methods 80 adult Sprague-Dawley rats were randomly divided into control group （ n = 40） and ANP group （ n = 40）. Sacrifices were made in 8,12, 16 and 20 h later in every group after ANP model was established by injecting 5 % sodium taurocholate into pancreatic duct respectively. The intestinal mucosal permeability and the expression of substance P were studied. Results In ANP groups, intestinal mucosal permeability and the expression of substance P were significantly higher than those in the control group （ P 〈 0.05）. The overexpression of substance P was correlated with the intestinal mucosal permeability in ANP（ r = 0.66, P 〈 0.01 ）. Conclusion The expression of substance P and the permeability in intestine of rats are increased significantly in ANP. There exists a possitive correlation between substance P and the intestinal permeability.
出处
《东南大学学报（医学版）》
CAS
2005年第6期359-363,共5页
Journal of Southeast University(Medical Science Edition)
基金
国家人事部留学回国人员科技活动择优资助经费资助项目(7690004027)
关键词
急性坏死性胰腺炎
神经激肽-1受体
P物质
肠黏膜通透性
大鼠
acute necrotizing pancreatitis
neurokinin-1 receptor
substance P
intestinal mucosal permeability
rats
