摘要
以进口纯人促甲状腺激素（ｈ—ＴＳＨ）作免疫原，经用Ｇａｌｆｒｅ等杂交瘤制备法，分别制备成１３Ｂ４、１３Ｃ４、８Ｈ１０、３Ｅ４四株具有抗ｈ—ＴＳＨ特异性，与促黄体素、促卵泡素、绒毛膜促性腺素无明显交叉反应。具有稳定分泌功能的杂交细胞株。经亚型鉴定均为ＩｇＧ１。液相中与１２５Ｉ—ｈＴＳＨ的最大结合率依次为１３Ｂ４、３Ｅ４、８Ｈ１０和１３Ｃ４，分别为６１％、５９％、５６％和３４％。表位分析表明，４株单抗主要针对两个不同表位。在此基础上已成功地建立ｈ—ＴＳＨ固相免放和酶免吸附超敏测定法。
Four hybridoma cell lines-13B4,3E4,8H10 and 13C4 are established while purified human thyrotropin is used as immunogen,The monoclonal antibodies produced are highly specific for two separately located epitopes of h-TSH and are all IgG1. Little or no cross-reaction with h-LH,h-FSH and hCG is observed. Binding capacities of the antibodies with125I-hTSH in liquid phase are 61%(13B4),59%(3E4),56%(8H10) and 34%(13C4). With those antibodies,uItra-sensitive IRMA and ELISA for h-TSH have been established.
出处
《上海第二医科大学学报》
CSCD
1996年第3期159-161,共3页
Acta Universitatis Medicinalis Secondae Shanghai
