摘要
目的探讨电针“内关”穴抗心肌缺血损伤的作用机理。方法取清洁级日本大耳白兔40只,按随机数字表法分为模型对照组10只;纳络酮对照组10只;电针加脑脊液组10只;电针加纳络酮组10只。观察下丘脑室旁核(PVN)微量注射纳络酮后电针“内关”穴对心肌缺血家兔心肌超微结构、Ⅱ导联心电图及心肌细胞跨膜电位的影响。结果PVN纳络酮微量注射对正常家兔心肌功能无影响,对缺血心肌功能影响轻微,在结扎(缺血)60min时发挥一定的保护作用(P<0·05);电针“内关”穴对缺血心肌有明显的保护作用,该作用可被PVN注射纳络酮所削弱(P<0·05),但不能完全阻断。结论(1)PVN在电针“内关”穴抗心肌缺血效应过程中发挥了重要作用;(2)PVN内阿片肽参与介导了“内关”穴抗心肌缺血的针刺效应;(3)除PVN及其内阿片肽外,PVN内的其他递质和中枢其他核团也可能在电针“内关”穴抗心肌缺血效应中发挥作用。
Objective To explore the mechanism of electroacupuncture at “Neiguan”（PC 6） antagonizing myo- cardial ischemia injury. Methods Forty rabbits were randomly divided into 4 groups, model control group, naloxone control group, electroacupuncture plus cerebral spinal fluid group, and electroacupuncture plus naloxone group, 10 rabbits in each group. The effects of electroacupuncture at “Neiguan” （PC 6） after injection of naloxone into the paraventricular nucleus （PVN） of the hypothalamus on myocardial ultrastructure, Ⅱ lead ECG and trasmembrane potentials of the ischemic myocardial cell in the rabbit of myocardial ischemia were investigated. Results The injection of naloxone into PVN has no effect on myocardial functions in the normal rabbit and a little effect on ischemic myocardial functions, but at 60 min after myocardial ischemia, this has a certain protective action on myocardial functions （P〈0.05）; electroacupuncture at “Neiguan” has obvious protective action on ischemic myocardium, which can be reduced by injection of naloxone into PVN （P〈0.05）, but can not be blocked. Conclusion （1） PVN plays an important role in the process of antagonizing myocardial ischemia by electroacupuncture at “Neiguan”; （2） endogenous opioid pepetides in PVN are involved in the acupuncture effect of antagonizing myocardial ischemia; （3） other transmitters in PVN and other nucleus groups in the center also can produce obvious effects in the antagonism to myocardial ischemia by electroacupuncture at “Neiguan”.
出处
《中国针灸》
CAS
CSCD
北大核心
2005年第10期720-724,共5页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金资助项目:30171179
关键词
电针
心肌缺血/针灸疗法
阿片样肽类/针灸效应
下丘脑室旁核/针灸效应
穴
内关
Electroacupuncture
Myocardial Ischemia/acup-mox ther
Opioid Peptides/acup-mox elf
Paraventricular Hypothalamic Nucleus/acup-mox elf
Point, PC 6 （Neiguan）
