摘要
目的:比较6种中药提取物(甲基莲心碱、粉防己碱、葛根素、灯盏花乙素、人参皂苷Rg1和人参皂苷Rb1)松弛离体新西兰白兔阴茎海绵体的作用.方法:采用离体新西兰白兔阴茎海绵体肌条张力记录法,观察6种中药提取物对去氧肾上腺素(PE)诱导收缩的阴茎海绵体肌条的松弛作用.结果:在(10-8～10-3)mol/L,甲基莲心碱、粉防己碱、葛根素和灯盏花乙素对PE诱导收缩的肌条具有浓度依赖性松弛作用,其IC50分别为4.60×10-6、3.73×10-5、8.03×10-4和3.33×10-3moL/L.而人参皂苷Rg1和人参皂苷Rb1对PE诱导收缩的肌条松弛作用较弱,终浓度为10-3mol/L时,仅分别松弛(16.32±13.36)%和(11.21±7.59)%.结论:6种中药提取物对离体新西兰白兔阴茎海绵体的松弛作用,甲基莲心碱最强.
Objective： To study the relaxats effects of six extractions from Chinese Herbs（ neferine, tetrandrine, kakonein, scutellafin, ginsenoside Rg1 and ginsenoside Rb1 ） on the corpus cavemosum tissue of rabbit in vitro. Methods ： Isolated stripes of rabbit corpus cavernosum tissue were precontracted with 10^-5 mol/L phenylephine（PE）. Relaxation in response to cumulative doses of six extracts at（ 10^-8～10^-3） mol/L was determined. Results： On rabbit cavemosal muscle stripes precontracted with PE, neferine, tetrandrine, kakonein and scutellarin showed dose dependent relaxation. IC50 values were 4.60 × 10^-6, 3.73 × 10^-5, 8.03 × 10^-4 and 3.33 × 10^-3mol/L, respectively. However, in the meantime, it was found that the relaxant effects of ginsenoside Rg1 and ginsenoside Rb1 less significant to stripes precontracted with PE. When the final concentration was 10^-3mol/L, the relaxations were only （16.32±5.45） % and （11.21 ± 3.10） %. Conclusion： Among the six extracts which showed relaxant effects to rabbit cavemosal muscle stripes precontracted with PE, neferine had greater functions than the other five extracts.
出处
《中华男科学杂志》
CAS
CSCD
2005年第10期793-795,799,共4页
National Journal of Andrology
基金
国家自然科学基金资助(30471736)
