摘要
自2002年水稻基因组测序完成后,水稻功能基因组学研究正在成为水稻研究的重要内容。构建突变体库是研究功能基因组学的一条重要而有效的途径。利用外源的Ac/Ds(Activator/Dissociation)标签系统是构建插入突变体库较为理想的方法,经过多年发展完善,其在水稻中已有广泛的应用,但仍面临着一些需要解决的实际问题。文章对Ac/Ds标签系统的转座行为及其构建突变体库的问题和优点进行了综述,总结了近年来Ac/Ds标签系统在水稻中的研究进展,分析了利用Ac/Ds标签系统进行功能基因组学研究所面临的挑战。
With the completion of the rice genome sequence in 2002, the study on functional genomics in rice has become a major task. Establishment of rice mutant library is an essential approach for rice functional genomics study. At present, utilizing maize transposable element Ac/ Ds （Activator/Dissociation） is a promising method to construct insertional mutagenesis library of rice. Ac/Ds tagging system has received extensive application in rice during the past several years, but it is still confronted with practical problems. In this paper, constructing rice insertional mutagenesis library using Ac/Ds-tagging system and transpositional behaviors of an Ac/Ds-tagging system, difficulties and advantages are reviewed. The research advances and challenge in rice functional genomics study using Ac/ Ds tagging system are also discussed and summarized in the paper.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2005年第5期441-450,共10页
Journal Of Plant Physiology and Molecular Biology
基金
国家自然科学基金项目(No.30471072)资助~~
关键词
水稻
Ac/Ds标签系统
插入突变体库
转座行为
功能基因组学
rice
Ac/Ds tagging system
insertional mutagenesis library
transpositional behavious
functional genomics
