摘要
目的研究丝裂霉素C-磁性纳米球胶体溶液剂和丝裂霉素C生理盐水溶液中丝裂霉素C(MMC)在小鼠体内的分布。方法建立生物样品中MMC的HPLC测定法,并测定了小鼠给药后的血浆及组织中药物浓度。结果小鼠尾静脉注射(1 mg.kg-1)丝裂霉素C-磁性纳米球胶体溶液剂,在外加磁场的引导下,30 m in即有82.72%的药物浓集于肝脏,是MMC生理盐水溶液34.83%分布量的2.37倍,在心、肾中的分布较MMC生理盐水溶液低。与尾静脉注射非磁丝裂霉素C纳米球胶体溶液剂的结果比较,外加磁场与磁纳米球的相互作用可大大地提高磁纳米球对肝脏的靶向率。与不施加磁场注射同剂量磁性纳米球的结果比较,说明外磁场可以非常有效地提高磁性纳米球在靶部位的浓集。结论丝裂霉素C制成磁性纳米球,在外加磁场的作用下具有很好的肝靶向性及一定的缓释和减毒效果。
Aim To study the distributive character of mitomycin （MMC） magnetic nanoparticles and MMC normal saline solution in mice. Methods A HPLC method for the determination of MMC in tissues and serum were established and applied to determine MMC in biological samples. Results Under the guidance of an external magnetic field, targeting rates of MMC to liver viscus are 82.72% after 30 minutes tail vein administration of （ 1 mg·kg^-1 ） mitomycin magnetic nanoparticles, it was 2.37 times larger than targeting rates of MMC normal saline solution group. The distributions of heart and kidney of mitomycin magnetic nanoparticles were less than that of MMC normal saline solution group. Compared with the results of tail vein administration mitomycin nanoparticles, interaction between the external magnetic field and magnetic nanoparticles is significantly effective to increase targeting rates of MMC to liver. Compared with the results of tail vein administration mitomycin magnetic nanoparticles without the external magnetic field, Mitomycin magnetic nanoparticles under the guidance of an external mag- netic field is significantly effective to increase targeting rates of MMC to liver. Conclusions Mitomycin magnetic nanoparticles under the guidance of an external magnetic field is significantly effective to increase targeting rates of MMC to liver and prolonging effect on the action in vivo.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第10期1187-1191,共5页
Chinese Pharmacological Bulletin
基金
国家高技术研究发展计划(863计划)重大专项基金资助项目(No2002AA214121)
广东省攻关项目(No2002C30103)
