期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

肌钙蛋白I2的原核表达及与ERRα1的体外相互作用 被引量：3

Prokaryotic Expression of Troponin I2 and Its in vitro Interaction with ERRα1
下载PDF
导出
摘要 孤儿受体雌激素受体相关受体α1(ERRα1)与乳腺癌有密切的关系.酵母双杂交筛选发现乳腺组织中肌钙蛋白I2(TNNI2)与其有明显的相互作用.为进一步研究TNNI2与ERRα1的相互作用,融合表达了GST-TNNI2原核蛋白,并体外翻译了35S标记的ERRα1蛋白,将二者共同温育进行GST捕获实验.放射自显影结果显示,TNNI2能够捕获35S标记的ERRα1,证明TNNI2与ERRα1存在体外直接的相互结合,提示了肌钙蛋白I2在ERRα1参与的生理过程中可能有重要的作用. Orphan receptor ERRal （estrogen receptor-related receptor al） is discovered to be closely linked with breast cancer. Distinct interaction between ERRα1 and TNNI2 （troponin Ⅰ2） in breast tissue was found by yeast two-hybrid screening. To further study the interaction between TNNI2 and ERRα1, fusion protein of GST-TNNI2 was expressed. ERRal protein labeled with 35S was translated in vitro and GST pull down assay was performed by incubating these two proteins together. Result of autoradiography showed that TNNI2 could pull down ERRal labeled with 35S. The result demonstrates that TNNI2 can bind to ERRal directly, and suggests that TNNI2 may play a crucial role in the physiological process of ERRα1.
作者 李渝萍 陈敏 陈彬 陈健 李强 周度金
机构地区 第三军医大学基础医学部生物化学与分子生物学教研室
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2005年第5期656-660,共5页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家自然科学基金(No.30370317 No.30300126) 第三军医大学创新基金(No.2458)资助~~
关键词 肌钙蛋白Ⅰ 雌激素受体相关受体α1(ERRα1) 蛋白质一蛋白质相互作用 troponin Ⅰ, estrogen receptor-related receptor al （ERRal）, protein-protein interaction
分类号 Q51 [生物学—生物化学] Q78 [生物学—分子生物学]
  • 相关文献

参考文献11

  • 1Margueron R, Duong V, Castet A, Cavailles V. Histone deacetylase inhibition and estrogen signalling in human breast cancer cells. Biochem Pharmacol, 2004, 68(6): 1239 ～ 1246.
  • 2Giguére V, Yang N, Segui P, Evans R M. Identification of a new classof steroid hormone receptors. Nature, 1988, 331(6151) :91 ～ 94.
  • 3Johnston S D, Liu X, Zuo F, Eisenbraun T L, Wiley S R, Kraus R J, Mertz J E. Estrogen-related receptor alpha 1 functionally binds as a monomer to extended half-site sequences including ones contained within estrogen-response elements. Mol Endocrinol, 1997, 11(3): 342 ～ 352.
  • 4Yang C, Zhou D J, Chen S. Modulation of aromatase expression in the breast tissue by ERR alpha-1 orphan receptor. Cancer Res, 1998, 58(24): 5695 ～ 5700.
  • 5陈彬 陈敏 等.核受体辅调节子结构与功能[J].第三军医大学学报,2001,23(1):149-151.
  • 6李渝萍,陈敏,陈彬,陈健,李强,周度金.利用酵母双杂合系统筛选与hERRα1相互作用的蛋白质[J].第三军医大学学报,2003,25(15):1352-1354. 被引量：5
  • 7陈健,陈敏,陈彬,李渝萍,李强,周度金.视黄醇结合蛋白RBP4可与多种核受体相互作用[J].中国生物化学与分子生物学报,2004,20(5):696-701. 被引量：9
  • 8Ni C Y. Cardiac troponin I: a biomarker for detection and risk stratification of minor myocardial damage. Clin Lab, 2001, 47(9-10):483 ～ 492.
  • 9Moses M A, Wiederschain D, Wu I, Fernandez C A, Ghazizadeh V, Lane W S, Flynn E, Sytkowski A, Tao T, Langer R, Troponin I is present in human cartilage and inhibits angiogenesis. Proc Natl Acad Sci USA, 1999, 96(6): 2645 ～ 2650..
  • 10Perry S V. Troponin Ⅰ: Inhibitor or facilitator. Mol Cell Biochem, 1999, 190(1-2): 9 ～ 32.

二级参考文献27

  • 1[1]Noy N. Retinoid-binding proteins: mediators of retinoid action.Biochem J, 2000, 348(3): 481 ～ 495
  • 2[2]Vanitallie T B. Frailty in the elderly: contributions of sarcopenia and visceral protein depletion. Metabolism, 2003, 52: 22 ～ 26
  • 3[3]Giguere V, Yang N, Segui S, Even R M. Identification of a new class of steroid hormone receptors. Nature, 1988, 331: 91～ 94
  • 4[8]Sambrook J, Russell D W. Molecular Cloning:A Laboratory Manual, 3rd ed. New York:Cold Spring Harbor Laboratory Press, 2001
  • 5[9]Biesalski H K, Nohr D. Importance of vitamin-A for lung function and development. Mol Aspects Med, 2003, 24(6): 431～440
  • 6[10]McKenna N J, Lanz R B, O' Malley B W. Nuclear receptor coregulators: cellular and molecular biology. Endocrine Rev, 1999,20(3): 321～344
  • 7[11]Hong H, Kohli K, Garabedian M J, Stallcup M R. GRIP-1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid and vitamin D receptors. Mol Cell Biol,1997, 17(5): 2735 ～ 2744
  • 8[12]Zhou D, Quach K M, Yang C, Lee S Y, Pohajdak B, Chen S. PNRC:a proline-rich nuclear receptor coregulatory protein that modulates transcriptional activation of multiple nuclear receptors including orphan receptors SF1 ( steroidogenic factor 1 ) and ERRalphal ( estrogen related receptor alpha-1). Mol Endocrinol, 2000, 14(7): 986 ～ 998
  • 9Squire JM et al. FASEB J, 1998, 12: 761-771
  • 10Perry SV. Mol Cell Biochem, 1999, 190: 9-32

共引文献18

同被引文献33

  • 1史建全,祁洪芳,杨建新.青海湖自然概况及渔业资源现状[J].淡水渔业,2004,34(5):3-5. 被引量：16
  • 2陈健,陈敏,陈彬,李渝萍,李强,周度金.视黄醇结合蛋白RBP4可与多种核受体相互作用[J].中国生物化学与分子生物学报,2004,20(5):696-701. 被引量：9
  • 3杨建新,祁洪芳,史建全,陈大庆,黄福祥,唐洪玉.青海湖水化学特性及水质分析[J].淡水渔业,2005,35(3):28-32. 被引量：22
  • 4李渝萍,陈彬,陈敏,陈健,李强,周度金.肌钙蛋白I2辅助活化多种核受体的反式作用[J].中国生物化学与分子生物学报,2005,21(6):763-769. 被引量：1
  • 5陈彬 陈敏 等.核受体辅调节子结构与功能[J].第三军医大学学报,2001,23(1):149-151.
  • 6Giguére V, Yang N, Segui P, Evans R M. Identification of a new class of steroid hormone receptors. Nature, 1988, 331(6151) :91 ～ 94.
  • 7Yang C, Zhou D J, Chen S. Modulation of aromatase expression in the breast tissue by ERR alpha-1 orphan receptor. Cancer Res, 1998,58(24) :5695 ～ 5700.
  • 8Johnston SD, Liu X, Zuo F, Eisenbraun T L, Wiley S R, Kraus R J,Mertz J E. Estrogen-related receptor alpha 1 functionally binds as a monomer to extended half-site sequences including ones contained within estrogen-response elements. Mol Endocrinol, 1997,11 (3): 342 ～ 352.
  • 9Zhou D, Quach K M, Yang C. PNRC: a proline-rich nuclear receptor coregulatory protein that modulates transcriptional activation of multiple nuclear receptors including orphan receptors SF1 (steroidogenic factor 1) and ERRalphal (estrogen related receptor alpha-1 ). Mol Endocrinol, 2000,14 (7) :986 ～ 998.
  • 10Zhou D, Chen S. PNBC2 is a 16 kDa coactivator that interacts with nuclear receptors through an SH3-binding motif. Nucleic Acids Res,2001,29 (19) :3939 ～ 3948.

引证文献3

二级引证文献3

中国生物化学与分子生物学报
2005年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部