摘要
目的研究深低温冷冻与环氧乙烷处理同种异体皮质骨板移植重建山羊股骨骨折的治疗效果.方法将16只山羊造成右侧股骨骨折,双侧股骨分别进行深低温冷冻与环氧乙烷处理同种异体皮质骨板移植,在术后3、6、12、24周处死动物取材,通过X线片,血管墨汁灌注,四环素荧光标记,组织学检查观察骨折愈合以及骨板存活情况.结果未骨折一侧术后3周,骨折一侧术后6周移植骨板出现再血管化.术后3～6周,骨折一侧移植骨板四环素荧光标记比未骨折一侧弱;而6周以后骨折一侧明显增强.术后12周骨折愈合,移植骨板与宿主骨骨性连接.术后24周移植骨板和宿主骨融为一体,骨折一侧移植骨板再塑性能力强,股骨皮质厚度增加明显.结论同种异体皮质骨板移植具有促进骨折愈合作用,移植骨板与宿主骨整合后能增加局部骨量储备,增强股骨强度.
Objective To study the outcome of reconstructing goat femoral fracture by use of cortical bone plates allografts that have been kept by deep-freezing at -70℃ for 4 weeks after being treated with 48℃ ethylene oxide. Methods The recipients, sixteen 10-12-month-old goats with fractures of right femur were subjected to the operation for transplanting the cortical bone plates allografts in the medial, lateral and back sides of fractured femurs. The goats were sacrificed and the specimens were procured at 3, 6, 12, and 24 weeks after surgery for X-ray photography, Chinese ink perfusion, tetracycline fluorescence labeling and histological observation in order to evaluate the healing of fracture and the incorporation of cortical bone plates allografts. Results The allograft strut was found revascularized at 6 weeks after surgery in the fracture group, whereas at 3 weeks in the control group. The tetracycline fluorescence labeling was poor in the fracture group as compared with that in the control group from 3 weeks to 6 weeks, but it was better in the fracture group than in the control group beyond 6 weeks after surgery. Fracture was healed and bone conjunction between allograft strut and host bone was seen at 12 weeks after operation. The allograft strut was incorporated in host bone, the ability of remodeling of allograft strut and the size of femoral cortex were better in the fracture group than in the control group at 24 weeks after surgery. The fracture was displaced in 19% animals and the allograft bone plates were not fractured. Conclusion The use of allograft strut pre-treated by ethylene oxide sterilization and deep-freezing could underpin fixation and promote healing of femoral fracture, and it can increase bone reservation and augment the strength of femur once the allograft strut is incorporated in the host bone.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第6期880-884,共5页
Journal of Sichuan University(Medical Sciences)
关键词
移植
同种
骨板
股骨骨折
Transplantation, homologous
Bone plates
Femoral fractures
