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PF40的亚细胞定位研究 被引量：4

Study of Subcellular Localization of PF40
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摘要 pf40基因是从谷子未成熟种子cDNA文库中获得的,与水稻、拟南芥的离子通道蛋白同源性很高,具有8个跨膜区.该基因转入烟草,可使烟草分枝增多,将其转入谷子,可使谷子分蘖增多.构建pf40与绿色荧光蛋白gfp的融合基因,转入烟草进行稳定表达,通过荧光显微镜和激光共聚焦显微镜观察研究其亚细胞定位,发现荧光主要集中在内质网.将pf40不同缺失区段的4个片段与gfp构建融合基因,转入烟草进行稳定表达,发现PF40N端93个氨基酸残基就能够使得PF40定位在内质网,C端缺失没有影响蛋白质的亚细胞定位. pf40 is obtained from millet unmature seeds cDNA library. It has high similarity with mental transporter family by blasting in DDBJ, GENBANK, EMBL. It is predicted that PF40 is included in ZIP family. Software analysis indicates that PF40 is a membrane protein. But which membrane system it localized in is unclear. A full PF40 cDNA fused at the 5＇ end of the green fluorescent protein （GFP） coding sequence was introduced and stably expressed in tobacco by agrobacterium-mediated transformation. The subcellular localization of GFP was analyzed by fluorescence microscope and laser confocal microscope. The fluorescence of PF40-GFP fusion protein was detected mainly in the ER, demonstrating that PF40 was mainly localized in the ER. 4 truncated pf40 fragments were fused with gfp. The truncated fusion genes also were introduced and stably expressed in tobacco. N-terminal amino acid region can locate the protein in the ER.
作者 夏玉凤 赵倩 于静娟 敖光明
机构地区 中国农业大学农业生物技术国家重点实验室
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2005年第11期1020-1025,共6页 Progress In Biochemistry and Biophysics
关键词 PF40/绿色荧光蛋白 定位 分枝 内质网 PF40/GFP, localization, branching, ER
分类号 Q7 [生物学—分子生物学]
  • 相关文献

参考文献19

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二级参考文献4

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共引文献5

同被引文献68

  • 1严建兵,汤华,黄益勤,郑用琏,李建生.玉米和水稻重要性状QTL的比较研究[J].Acta Genetica Sinica,2004,31(12):1401-1407. 被引量：18
  • 2夏玉凤.以gfp为报告基因研究蛋白亚细胞定位[J].生物技术通报,2006,22(2):11-13. 被引量：8
  • 3石乐义,李美茹,李洪清,陈贻竹.植物Na^+/H^+逆向转运蛋白功能及调控的研究进展[J].广西植物,2006,26(6):602-607. 被引量：8
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生物化学与生物物理进展
2005年 第11期

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