摘要
采用药敏纸片法对新狼毒素B(化合物1)及其羟基取代产物(化合物2)、羰基还原产物(化合物3)和狼毒色原酮(化合物4)、青霉素钠(化合物5)、头孢哌酮钠(化合物6)进行抑菌活性检测和比较.结果发现,化合物1和化合物2对金黄色葡萄球菌、无乳链球菌、乳房链球菌、停乳链球菌、大肠杆菌5种供试菌均有不同程度的抑菌活性,即二者对革兰氏阳性菌和阴性菌均有活性,其中化合物1较化合物2的活性更强.化合物1～化合物5对4种球菌的抑菌活性大小次序为5＞1＞3＞4＞2.化合物1～化合物4、化合物6对大肠杆菌的活性大小为6＞3＞1＞2＞4.从而初步得出新狼毒素B分子中羟基为主要活性基团,另外,其经过羰基还原后虽然对4种球菌的抑菌活性有轻微的降低,但是对大肠杆菌的活性增强了,也就是说其抑菌活性的选择性提高了.
The antibacterial activity of the hydroxyl substitution product（compound 2） and carbonyl disoxidation product（compound3） of neochamaejasmine B,chamaechromone（compound 4） and neochamaejasmine B（compound 1 ,penicillin Na（compound 5） and Ceftriaxone Sodium（compound 6） were tested by dish diffusion of Neo-Sensitab. The result showed that the compound 1 and compound 2 had different antibacterial activity in its degree on S. aureas, S. agalactiae ,S. uberis, S. dysagalactiae and E. coll. That is, the two compounds have antibacterial activity on Gram-positive bacterium and Gram-negative bacterium, moreover, the activity of the compound 1 is stronger than compound 2. The sequence of antibacterial activity of compound 1 to compound 5 is that： 5〉1〉3〉4〉2 and that of compound 1 to compound 4 and compound 6 is that.6〉3〉1〉2〉4. The above mentioned showed that the hydroxyl of neochamaejasmine B was the major active situs. Furthermore, the bacterial activity of carbonyl disoxidation product on E. coli strengthen although that of it on rotor cocci was slightly lower, that is, selectivity of its bacterial activity increased.
出处
《动物医学进展》
CSCD
2005年第11期66-69,共4页
Progress In Veterinary Medicine
基金
国家自然科学基金(30571402)
国家经贸委重点技术创新项目(02CJ-05-01-41)
陕西省科技攻关项目(2003K-G2-02)
西北农林科技大学青年基金重点项目(080807)
关键词
抗菌活性
构效关系
新狼毒素B
狼
毒色原酮
antibacterial activity
structure-activity relationship
neochamaejasmine B
chamaechromone
