摘要
目的:探讨骨质疏松大鼠骨生物力学的变化情况及其硝酸镓对骨质疏松的干预作用。方法:实验于2003-06/12在西安交通大学医学院教育部重点实验室完成。选择成年雌性SD大鼠66只,随机分2组,骨质疏松模型组43只,对照组23只。全部大鼠均经乌拉坦腹腔注射麻醉后开腹,对照组切一小块脂肪后缝合;骨质疏松模型组切除双侧卵巢后缝合(4只大鼠死于术后感染)。以常规饲料喂养12周后,每组随机选8只大鼠麻醉处死取胫骨,观察模型复制情况。确认模型复制成功后,进入治疗阶段。骨质疏松模型组剩余的31只大鼠分为2组,骨质疏松组和硝酸镓治疗组分别为15和16只,骨质疏松组正常饲养;硝酸镓治疗组给予1mg/kg硝酸镓,3次/周,腹腔注射,共注射12周。①取大鼠双侧后肢骨分别冰冻或固定。②骨钙、镓含量使用原子吸收分光光度法测定;骨磷含量用钼兰比色法测定。③测定骨质量,包括骨湿重、干重和灰重。④测定骨强度应用材料试验机作三点弯曲试验。结果:共选择20只大鼠进入骨微量元素、骨质量及骨强度测定实验,骨质疏松组8只,硝酸镓治疗组6只,对照组6只,其他大鼠用于本课题另一方面的实验。20只大鼠全部进入结果分析。①各组大鼠股骨中钙、磷、镓含量比较:各组骨钙、磷未见显著性变化,但硝酸镓治疗组大鼠骨镓含量显著高于对照组及骨质疏松组[(6.96±0.61,0.22±0.17,0.27±0.15)μg/g(t=-11.673,-13.568,P<0.01)]。②各组大鼠股骨强度比较:骨质疏松组大鼠股骨强度显著低于对照组和硝酸镓治疗组(t=19.770,31.216,P<0.01)。③各组大鼠股骨剥离后湿重、干重与体质量比值的比较:骨质疏松组大鼠股骨湿、干重与体质量之比值均小于对照组和硝酸镓治疗组[(0.00240±0.00014,0.00267±0.00021,0.00273±0.00008);(0.00165±0.00011,0.00190±0.00018,0.00197±0.00007)(t=-2.361,-2.473,2.187,2.300,P<0.05)]。结论:骨质疏松可引起大鼠骨生物力学强度降低,硝酸镓对骨质疏松引起骨强度降低有改善作用。
AIM： To investigate the changes of bone biomechanics in osteoporosis rats and the intervention effects of gallium nitrate on osteoporosis. METHODS： The experiment was conducted at the Key Laboratory, Department of Education, Medical College, Xian Jiaotong University between June and December 2003. Sixty-six adult female SD rats were divided into 2 groups at random： the osteoporesis model group with 43 rats and the control group with 23 rats. All the rats were treated with urethane, and then the abdomens were dissected after anesthesia with abdominal cavity injection. The rats in the control group were sutured after cutting out a block of fat; The rats in the osteoporosis model group were sutured after cutting out the ovaries at the two sides （Four rats died of postoperative infection）. After feed for 12 weeks with routine feed, 8 rats were selected from every group randomly, and killed to get the tibia. The duplication of models was observed. After the definition of success of copying models, we entered the therapy phase. The rest 31 rats in the osteoporosis model group were divided into 2 groups. There were 15 rats and 16 rats in the osteoporosis group and gallium nitrate therapy group, respectively. Those in the osteoporosis group were fed normally. Those in the gallium nitrate therapy group were treated with 1 mg/kg gallium nitrate, 3 times a week through abdominal cavity for 12 weeks totally. ① The hindlimbs of the double side in rats were gained to freeze or fix, respectively. ② Atomic absorption spectropbotometer was used to detect the contents of calcium and gallium. Molybdenum blue chromatometry was used to detect the contents of phosphorus. ③ The density of bone was detected, including wet weight, dry weight and grey weight. ④ Material test machine was used to determine the intensity of femurs by 3 points distortion test. RESUILTS： Totally 20 rats were collected into the test of bone microelement, density of bone and intensity of bone, 8 rats in the osteoporosis group, 6 in the gallium nitrate therapy group and 6 in the control group, and other rats were used in the other aspect of the experiment on this subject. Twenty rats were all involved in the result analysis.① Comparison of contents of calcium, phosphorus and gallium in the femurs in every group： There was insignificant change in the contents of calcium and phosphorus in every group, but the content of gallium in the gallium nitrate therapy group was significantly higher than that in the control group and osteoporosis group [（6.96±0.61,0.22±0.17,0.27±0.15）μg/g（t=-11.673,-13.568,P〈0.01）]. ② Comparison of intensity of femur in rats of every group： It was lower in the ostcoporosis group significantly than that in the control group and gallium nitrate therapy group （t=19.770, 31.216, P 〈0.01 ）. ③ Comparison of odds of wet weight, dry weight and body mass in rats of every group after the stripping of femur： It was less in the osteo- porosis group than those in the control group and gallium nitrate therapy group [（0.002 40±0.000 14,0.002 67±0.000 21,0.002 73±0.000 08）;（0.001 65±0.000 11,0.001 90±0.000 18,0.001 97±0.000 07）（t=-2.361,-2.473,2.187,2.300,P〈0.05）]. CONCLUSION： Osteoporosis can decrease the intensity of biomechanics of femurs.Gallium nitrate can lessen the decrease of bone intensity induced by iosteoporosis.
出处
《中国临床康复》
CAS
CSCD
北大核心
2005年第39期134-135,137,共3页
Chinese Journal of Clinical Rehabilitation
基金
兰州军区医药卫生科研基金项目资助(LXH20-11)~~
