摘要
为了观察体外培养的大鼠海马星形胶质细胞(ASTs)在红藻氨酸(KA)作用下的超微结构变化,我们体外培养大鼠乳鼠海马ASTs,在KA不同浓度(25μmol/L、250μmol/L)及时间(10min、100min)处理条件下,采用原子力显微镜(AFM)扫描细胞表面超微结构改变,透射电镜(TEM)观察细胞内部超微结构变化。结果表明,与对照组相比,低浓度KA(25μmol/L)处理组的细胞表面超微结构无明显改变,但胞内出现空泡样变,溶酶体增多;高浓度KA(250μmol/L)处理组的细胞表面有“孔洞”、“裂隙”样改变,直径在数百纳米,这些表现在KA长时间处理组(100min)更加明显;TEM下可见溶酶体增多、胞核扭曲,在KA长时间处理组甚至出现了自噬小体。以上结果说明培养的ASTs经KA处理后能发生一些损伤性的超微形态结构变化,且具有剂量及时间依赖性,而AFM观察到的KA处理使细胞膜出现“孔洞”、“裂隙”样变化可能是不可逆性兴奋性神经毒性损伤的结构基础。
In order to observe the ultrastructured changes of cultural rat hippocampal astrocytes in respond to kainic acid（KA）exposure,the rat hipporampal astrocytes were treated with KA at different concentrations（25μmol/L,250μmol/L）for different durations（10min,100min）,Then,the ultratstructural changes of the astrocyte were seaned by the atomic force microscope（AFM）and observed by transmission clectrou microscope（TEM）,respectively,The results showed that compared with control group,there were no distinguished ultrastructrual changes of the membrane surface when the cells exposed to the 25 μmol/L KA,but in the higher concentration group （250μmol/L）,some changes,such as“holes”and “canyons”in the membrane were seen,and these changes were especialy predominant after the duration extended to 100 min,In the low concentration group,TEM observation had found some ultrastructural changes,such as vacuolar degeneration,mitochondrial swelling,and large number of lysosomes.Even some twisted nucleus and autophagosomes were observed in the 250μmol/L KA-100 min group,The present results indicate that KA treatment on cultured rat hippocampal astrocytes could induce ultrastructural changes and these morphologic changes have a dosage-time dependence.The“holes”and “canyons” showed by AFM observation might be the pathologic morphologic bases of the irreversible neurotoxicity.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2005年第6期615-621,共7页
Chinese Journal of Neuroanatomy
