摘要
A new electrochemical method for the on-line determination of vitamin B1 is presented. Based on dead-stop endpoint biamperometry by using two platinum foil electrodes with an applied potential difference of 150 mV, vitamin B1 can be oxidized by hexacyanoferrate （ Ⅲ ） in a sodium hydroxide medium via a reversible indicating couple Fe（ CN）6^4- /Fe（CN）6^3- The cell current is linear with the concentration of vitamin B1 in the concentration range of 4. 0 × 10^-6-1.0 × 10^ -3 mol/L with a detection limit of 8.0 ×10 ^-7 mol/L（0. 27 μg/mL）. Most familiar excipients, ions and vitamins do not interfere with the determination of vitamin B1. The method displays the advantages of simplicity, high efficiency（ 180 samples/h）, and high selectivity, and is suitable for the determination of vitamin B1 in pharmaceutical preparations.
A new electrochemical method for the on-line determination of vitamin B1 is presented. Based on dead-stop endpoint biamperometry by using two platinum foil electrodes with an applied potential difference of 150 mV, vitamin B1 can be oxidized by hexacyanoferrate （ Ⅲ ） in a sodium hydroxide medium via a reversible indicating couple Fe（ CN）6^4- /Fe（CN）6^3- The cell current is linear with the concentration of vitamin B1 in the concentration range of 4. 0 × 10^-6-1.0 × 10^ -3 mol/L with a detection limit of 8.0 ×10 ^-7 mol/L（0. 27 μg/mL）. Most familiar excipients, ions and vitamins do not interfere with the determination of vitamin B1. The method displays the advantages of simplicity, high efficiency（ 180 samples/h）, and high selectivity, and is suitable for the determination of vitamin B1 in pharmaceutical preparations.
基金
SypportedbyGuangxiYoungScienceFoundation(No.0135003)andMiddleYoungScienceScholarLeaderFoundationofGuangxiUniversities.
