摘要
应用3H-胸腺嘧啶核苷(3H-TdR)掺入及液体闪烁测量技术,观察不同浓度的阿霉素,5-氟尿嘧啶(5-Fu)、三尖杉酯碱及去炎松对体外培养的人视网膜色素上皮细胞(RPE)增殖的抑制作用。结果;阿霉素在2~32ng/ml时,对RPE细胞抑制率为85~77.2%,ID50为12ng/ml;5-Fu在0.2~3.2μg/ml时,对RPE细胞抑制率为14.5~81.7%,ID50为0.6μg/ml;三尖杉酯碱在2.5~40ng/ml时,抑制率为18.5~94.1%,ID50为4.5ng/ml;去炎松在200~350μg/ml时对RPE细胞抑制率为37.9~53.5%,ID50为340μg/ml。结论:阿霉素、5-Fu、三尖杉酯碱及去炎松能有效地抑制RPE细胞的增殖。
This study was to select drugs against cellular proliferation in the vitreous. Doxorubicin (2ng/ml-32ng/ml), fluorouracil (5-Fu) (0. 2ug/ml-3. 2ug/ml), harringtonine (2. 5ng/ml-40ng/ml ) and triacinrolone acetonide(TA) (150ug/ml-350ug/ml) were added to cultures of human retinal pigment epithelial(RPE) cells respectively and proliferation rates of the cells were measured with 3H-thymidine incorporation and liquid scintillation techniques on days. The results showed that doxorubicin, 5-Fu, harringtonine and TA inhibited cellular proliferation effectively and in a dose-dependent manner (doxorubicin, 8. 7-77. 5% ;5-Fu, 14. 5-81. 7%;harringtonine, 18. 5-94. 1%; TA, 18-53. 5% P<0.05). Their ID were 12ng/ml 0. 68ugg/ml, 4. 5ng/ml, and340ug/ml, respectively. Doxorubicin, 5-Fu,harringtonine and TA had an effective inhibition on human RPEcellular proliferation.
出处
《眼科研究》
CSCD
1996年第1期22-24,共3页
Chinese Ophthalmic Research
关键词
视网膜病变
色素上皮细胞
阿霉素
氟尿嘧啶
retinal pigment epithelium
doxorubicin
5-fluoroucil
harringtonine
triactinrolone acetonide