摘要
目的研究trichostatin A(TSA)诱导肺癌细胞系SPC-A-1细胞凋亡。方法 MTT法测定TSA对SPC-A-1的细胞毒性,荧光显微镜观察细胞DNA的变化,琼脂糖凝胶电泳分析细胞凋亡,流式细胞仪分析细胞周期及线粒体膜电位的变化。结果 TSA对SPC-A-1细胞的IC_(50)为3．36μg／mL。SPC-A-1细胞生长曲线表明,TSA浓度增高,细胞生长率明显下降,细胞凋亡可在1～16μg／mL TSA处理后24h出现。凋亡细胞主要表现为核染色质固缩,荧光染色增强,琼脂糖凝胶电泳图谱呈现“梯子状”电泳。TSA阻断细胞于G_1期。线粒体膜电位降低。结论 YSA可以诱导SPC-A-1细胞发生凋亡,途径可能是通过线粒体旁路。
Objective To investigate the apoptosis of SPC-A- 1 cells induced by trichostatin A（TSA）. Methods Cytotoxicity and cell viability were assayed by MTT method. Morphologic assessment of apoptosis was performed with fluorescence microscope, cell cycle and mitochondrial membrane potential were analyzed by flow cytometry, DNA ladder was detected by agarose gel electrophoresis. Results SPC-A-1 cells treated with TSA showed apparently cytotoxicity, IC50 of TSA was 3.36μg/mL. The growth curve showed the ratio of growth decreased with the increase of concentration of TSA. The apoptosis appeared 24 hours after treated by 1 16 μg/mL TSA, morphologic changes including nuclear chromatin condensation fluorescence strength were observed with fluorescence microscope and DNA gel electrophoresis showed ladder. Conclusion TSA can induce SPC-A-1 cell apoptosis by mitochondria pathway.
出处
《肿瘤》
CAS
CSCD
北大核心
2005年第6期530-533,共4页
Tumor
基金
广东医学院青年基金(编号:200104/K01060)
