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Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells 被引量:10

Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells
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摘要 AIM: To study the function of α-fetoprotein (AFP) in SMMC-7721 hepatoma cells.METHODS: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescenceactivated cell sorter (FACS).RESULTS: The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis.CONCLUSION: Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis. AIM: To study the function of α-fetoprotein (AFP) in SMMC- 7721 hepatoma cells. METHODS: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescenceactivated cell sorter (FACS). RESULTS: The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis. CONCLUSION: Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis.
出处 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第38期6053-6055,共3页 世界胃肠病学杂志(英文版)
基金 Supported by the Natural Science Foundation of Shandong Province, No. Z2003C01
关键词 SIRNAS AFP SMMC-7721 cell PROLIFERATION Apoptosis α-胎蛋白 siRNA 细胞增殖 SMMC-7721细胞
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