摘要
目的观察茶多酚对人舌鳞状细胞癌Tca8113细胞增殖和端粒酶催化亚基(hTERT)的影响。方法实验分0.025、0.050、0.100、0.200g/L茶多酚组和空白培养基组。采用噻唑蓝比色法检测茶多酚对Tca8113细胞增殖的影响,逆转录PCR法检测hTERT基因表达,Western-blot法测定细胞中hTERT蛋白表达量。所有数据采用ANOVA单因素方差分析和Student-Newman-Keuls检验进行统计学检验。结果茶多酚能明显抑制Tca8113细胞增殖,并存在时间和浓度依赖性。72h时,0.200、0.100、0.050和0.025g/L茶多酚组细胞增殖抑制率分别为69.75%±3.24%、63.17%±3.19%、50.35%±4.21%和34.75%±3.71%。72h时,0.100、0.050g/L组hTERT mRNA和蛋白表达均低于空白对照组(P<0.05)。结论茶多酚能够抑制人舌鳞状细胞癌Tca8113细胞株的增殖及端粒酶催化亚基的转录和表达,抑制端粒酶催化亚基可能是茶多酚抗舌癌的机制之一。
Objective To investigate the effects of tea polyphenols （TP） on cell proliferation and human telomerase reverse transcriptase （hTERT）. Methods The experiment was divided into tea polyphenols 0.100g/L , tea polyphenols 0. 050g/L and blank control groups. The inhibitory ratio of cell proliferation was assayed with MTT, and hTERT mRNA was detected by RT-PCR, and hTERT protein analyzed by Western-blotting. The data were analyzed by one-way ANOVA and Student-Newman-Keuls of SPSS 11.0 for windows. Results Cell proliferations were significantly inhibited after exposure to TP. The proliferation inhibiting rate of TP 0. 025, 0. 050, 0. 100,0. 200 g/L on Tca8113 cell at 72 h was 69. 75%±3.24%,63.17%±3.19%,50.35%±4.21% and 34. 75%±3.71%,respectively（F=270.19, P〈0. 05）. At the end of 72 h, the hTERT mRNA expression in TP 0. 100 g/L, 0. 050 g/L and control group were 0. 32±0. 05, 0.41±0. 04, 0. 72±0. 05, respectively（ P〈0.05 ）. The Western-blotting assay showed that hTERT protein was also decreased by tea polyphenols compared to control group. Conclusions Tea polyphenols could inhibit the proliferation of Tca8113 cells and expression of the hTERT mRNA and protein in Tca8113 cell lines. This effect might be one of the mechanisms for anticancer function of tea polyphenols.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2005年第6期451-454,共4页
Chinese Journal of Stomatology
关键词
癌
鳞状细胞
肿瘤细胞
培养的
茶多酚
Carcinoma, squamous cell
Tumor cells,cultured
Tea polyphenols
