摘要
目的通过观察大鼠视细胞光损伤模型的视觉运动行为,评价光感受器细胞的损伤、死亡和视功能的对应关系,以期建立评估动物视功能的方法。方法21只SD大鼠分为3组,暗适应24h后选用(950±50)流明绿色光分别照射3、12、24h,光照后暗适应24h,暗适应完成48h后摘除眼球,冷冻切片分别行HE、rhodopsin免疫荧光染色;以大鼠视觉意识障碍反应、前置刺激反应作为视功能的判断指标,分别于光损伤前、后2d测定大鼠的视觉运动行为,对SD大鼠光损伤前、后的平均运动速度作统计学分析。对照组为7只双眼球摘除术后的大鼠。结果视觉意识障碍反应、前置刺激反应结果表明,随着光照时间的延长,动物的视觉意识障碍反应逐渐丧失,前置刺激反应逐渐增强。统计学结果显示,SD大鼠的视觉运动行为变化是与视功能相关的。随着损伤程度的加重,SD大鼠视觉运动的平均运动速度出现渐进性下降的变化。结论随着视细胞损伤程度的加重,SD大鼠的视功能呈现渐进性的下降,其视觉运动的平均运动速度亦出现相应的变化。视觉运动行为测试可以用于对SD大鼠的视功能进行定量的评估。
Objective To evaluate the relationship of visual function and photoreceptor cell loss, and to establish a visual function assessment system based on the observation of optokinetic behavior in rat with photic injury. Methods SD rats were exposed to （950 ±50）lux green light for 3 hours, 12 hours and 24 hours respectively after 24-hour dark adaption. Then 24-hour dark recovery was followed by 48-hour observation before the eyes were enucleated. Frozen sections were obtained. Slides were stained by HE and rhodopsin immunofluorescent stainings. Vision mediated disturbance of consciousness and pre-pulse response were used to assess the visual function. Optokinetic behavior test was conducted 2 days before and after photic injury. Average velocity of motion were analyzed. Results Vision mediated disturbance of consciousness response are gradually weakened; while pre-pulse response is reinforced as the photic injury time increases. The relationship of optokinetic behavior and rat visual function is statistically significant. Optokinetic behavior test shows the average velocity of motion gradually decreases as the injury is aggravated. Conclusions Longer light exposure time leads to increased photoreceptor injury, which in turn, causes decrease of visual function in SD rat. Optokinetic behavior test, represented by average velocity of motion, is correlated with rat visual function. Thus, optokinetic behavior test can be used to assess the visual function of SD rat quantitatively. （Chin J Ophthalmol , 2005,41：924-929 ）
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2005年第10期924-929,共6页
Chinese Journal of Ophthalmology
基金
国家自然科学基金资助项目(30170999)
北京市科委资助项目(H020220010390)
