摘要
目的探讨胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-3(IGF-BP-3)在去势大鼠阴茎海绵体中的表达及其意义。方法取16只成年雄性大鼠随机分为去势组、对照组。1周后取阴茎海绵体, 比色法测定阴茎海绵体一氧化氮(NO)含量(ΜG／G);原位细胞凋亡标记检测细胞凋亡数;逆转录多聚酶链聚合反应检测IGF-1、IGF-BP-3MRNA表达。结果 IGF-1MRNA在对照组中表达(1．44±0．29)而在去势组未检测到表达。IGF-BP-3MRNA在去势组表达量(3．52±1．4)较对照组(1．10±0．51)升高(P<0．01)。去势组阴茎海绵体细胞凋亡数(26．02±5．25)较对照组(12．51±1．81)高(P<0．05);凋亡细胞积分光密度去势组(33931．54±2459．36)较对照组(18766．37±3040．42)高(P<0．05)。去势组阴茎海绵体 NO浓度(14．45±2．38)较对照组(39．8±3．28)显著降低(P<0．01)。结论去势1周后阴茎海绵体细胞发生凋亡。去势后阴茎海绵体细胞凋亡可能与IGF-1MRNA表达降低及IGF-BP-3MRNA表达增高有关。
Objective To elucidate the functional change of Insulin-Like Growth Factor-1（IGF-1） and Insulin- Like Growth Factor-binding Protein-3 （IGF-bp-3） of castrated Rat in corpus cavernosum.（RCC）. Methods Sixteen mature male rats were randomly divided into 2 groups：One-week castration and sham-operated（control）.One week after castration, corpus cavemosum were harvested. The expression of mRNA on IGF-1, IGF-bp-3 from 7-day castrated and control were detected by Reverse Transcriptase Polymerase Chain Reaction （RT-PCR）.Apoptosis was detected by using in situ Terminal-Dexynuleotidy Transferase-mediated duTp nick end labeling （TUNEL）; NO concentration in rats corpus cavemosum were measured by spectrophotometry. Results IGF-1 gene expression in the corpus cavemosum from control group （1.44±0.29）, the catstration group was not detected. Compared to control group （1.1±0.51）, IGF-bp-3 mRNA expression from the castration group （3.52± 1.4） was markedly increased （P 〈 0.05）. Compared to the control group （12.51 ± 1.81）, apoptosis extent of RCC from the castration group （26.02±5.25） was greatly increased （P 〈 0.05）. Compared to control group （39.8±3.28）, NO concentration in RCC from the castration group （14.45±2.38） was greatly decreased. Conclusion Castration can induce apoptosis in RCC. The down-regulated expression of IGF-1mRNA and up-regulated expression of IGF-bp-3mRNA are likely involved in the apoptosis of RCC after castration.
出处
《中国男科学杂志》
CAS
CSCD
2005年第6期1-3,7,共4页
Chinese Journal of Andrology
