摘要
目的研究颗粒物PM2.5对慢性支气管炎大鼠与正常大鼠的急性毒效应,以及这种毒效应在模型组和正常组之间的差异。方法采用气管滴注的方法,对慢性支气管炎模型组和正常组连续染毒3天,在最后一次染毒24小时后处死动物,分析肺灌洗液中白蛋白(ALB)、乳酸脱氢酶(LDH)、碱性磷酸酶(AKP)、丙二醛(MDA)和谷胱甘肽(GSH)的含量。结果颗粒物PM2.5对模型组和正常组动物均产生急性毒作用,且存在剂量反应关系;模型组肺灌洗液中ALB、LDH、AKP和MDA四项指标在高剂量组均明显高于正常组(P<0.05),而GSH低于正常组(P<0.05)。结论颗粒物PM2.5可以导致大鼠急性肺损伤,并且慢性支气管炎模型组比正常组对颗粒物PM更加易感。
Objective To study the acute toxicity of PM2.5 to the rats with chronic bronchitis and the control rats and the difference between the model rats and the control rats on PM2.5 toxicity. Methods With the use of an intratracheal instillation, both groups were exposed to suspensions of PM2.5 . After three times, the levels of ALB, LDH, AKP, MDA and GSH in bronchoalveolar lavage fluid （BALF） were analyzed. Results PM2.~ could induce the acute toxicity to both model rats and control rats, and dose-response relationships were observed in all indexes. In model rats, the levels of ALB, LDH, AKP, and MDA were higher in control rats（ P 〈 0.05）, while the levels of GSH were lower in control rats （P 〈 0.05）. Conclusion PM2.5 could have acute toxic effects on both model rats and health rats, and the rats with chronic bronchitis are more susceptible to PM2.5 than health rats.
出处
《卫生研究》
CAS
CSCD
北大核心
2005年第6期667-670,共4页
Journal of Hygiene Research
基金
国家自然科学基金资助项目(No.30271106)
关键词
PM2.5
慢性支气管炎模型
氧化损伤
易感性
PM2.5 , a rat model of chronic bronchitis, oxidative damage, susceptibility
