摘要
用异型双功能连接剂ＳＰＤＰ，将核糖体抑制蛋白一天花粉蛋白（ＴＣＳ）共价连接在单克隆抗体ＮＤ－１上，制成免疫毒素，此单抗与人大肠癌细胞表面抗原一大分子表面抗原（ＬＥＡ）有高度亲合能力。连接物ＮＤ－１－ＴＣＳ用ＳｅｐｈａｄｅｘＧ－１５０分子筛层析纯化。间接免疫荧光试验结果表明，ＮＤ－１－ＴＣＳ保留了较高的抗体活性。体外细胞毒性试验表明，ＮＤ－１－ＴＣＳ对人大肠癌细胞ＣＸ－１的ＩＣ＿（５０）。为０．３６μｇ／ｍｌ．细胞毒性ＮＤ－１和ＴＣＳ混合物高１０倍。ＮＤ－１－ＴＣＳ对人大肠癌细胞的细胞毒性比非抗原携带细胞ＳＰ＿（２／０）高６０倍。此免疫毒素在人大肠癌的治疗方面有应用前景。
ribosome inactivating protein-trichosanthin(TCS ) was covalently linked to a monoclonal anti-body ND-1to prepare a kind of immunotoxin by heterobifunctional reagent SPDP,which exhibited highaffinity for cell surface antigen large external antigen (LEA)of hurnan gut carcinoma cells. The conju-gant ND-1-TCS(Immunotoxins were purified on Sephadex G-150 column, The results of indirect im-munofluo-rescent test showed that ND-1-TCS was retained very high antibody activity. The in vitro cy-totoxicity assays showed ND-1-TCS killed human gut carcinoma CX-1 by 50%(IC_50)at concentrationof 0.36μg/ml.The cytotoxicity was 10-fold higher than that of the mixture of free TCS and ND-1.The cytotoxicity of ND-1-TCS to CX-1 cell was 60-fold higher than that of ND-1-TCS and TCS toLEA-negative carcinoma cell SP_2/0.The result indicated that the immunotoxins may be proved useful inthe treatment of human gut carcinoma.
出处
《中国医科大学学报》
CAS
CSCD
1996年第2期111-113,共3页
Journal of China Medical University
基金
"国家"八五"科技攻关项目
