摘要
本实验用电镜观察了弓形虫ＮＴ株在猴肾细胞、人胚肺细胞、恶性胶质瘤细胞及小鼠腹水、肝脏和子宫等组织中的生长繁殖形式，并计数各种繁殖方式出现的百分率，结果显示，大多数弓形虫以内芽殖方式繁殖，少部为等分裂繁殖，裂殖繁殖最少见。裂殖繁殖时弓形虫体内的子细胞生长发育常不是同步的。成熟的子细胞以出芽的方式脱离母体，并围绕母体形成玫瑰花状或蜂窝状等群落。三种繁殖方式在各种宿主细胞和组织中出现的频率各不相同，小鼠肝和子宫组织中弓形虫等分裂繁殖较在其它细胞中少见，裂殖繁殖仅见于培养细胞和小鼠腹水中。提示宿主细胞种类的不同可能影响弓形虫的繁殖方式。
The methods of reprodution undergone by virulent strain of T.gondii in human glioblastoma cells,human embryonic pulmonary cells, monkey kideny cells,pertoneal exudate,tissue of uterus and liver of mice were observed and quantified by electron microscopy.The results showed that most of the T.gondii reproduced by endogeny. The others multiplied mainly by binary fisson.Schizogony in T. gondii was seldom seen. Development and growth of each daughter cell of T.gondii was not synchronous during schizogony.After the daughter cells were mature, they budded off and surronunded the mother cell.Rosettelike and honeycomb-like colonies were formed.The relative frequeney of the three reproductive ways of T. gondii were different in each kind of host cells and tissues.Binary fission were fewer in liver and uterus of mice than in cultural cells and peritoneal exudate, in which schizogony was observed only. The results suggested that the ways of reproduction under gone by T. gondii should be probably influenced by the type of host cells.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1996年第3期12-15,共4页
Chinese Journal of Zoonoses
基金
国家自然科学基金
关键词
弓形体
繁殖
电镜
培养细胞
Toxoplasma gondii,Reproduction,Electron microscopy,Cultural cell,Mouse
