摘要
体外培养的人睾丸组织分为对照组、单剂人绒毛膜促性腺激素（hCG）组、隔3日双剂hCG组及连续多剂hCG组。在加入hCG（20kIU/L）后温育24小时,收集培养液,测定睾酮。结果表明3个hCG处理组给予第1剂hCG后48～72小时出现分泌高峰;间隔3日再次刺激仍可诱发睾酮合成增加,但峰值减低;而连续刺激则睾酮水平未增加。
Cultured human testicular tissues were grouped as follows:control group,group tracted with single dosc of human chorionic gonadotropin(hCG), group treated with double doses of hCG given at three-day-interval and group treated with multiple doses of hCG. The dose of hCG was 20kIU/L,and it was added into the medium and incubated for 24 hours. Testosterone was measured by RIA. The result indicated that all three hCG-treated groups had a testosterone secretion peak at 48-72 hours after the first dose of hCG. After the second hCG treatment given in three days interval the testosterone increased again. but its maximal rise reduced. Multiple doses of hCG given successively inhibited the response of Leydig cells to hCG stimulation.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1996年第1期28-30,共3页
Chinese Journal of Endocrinology and Metabolism
关键词
睾丸
睾酮
HCG
Human chorionic gonadotropin
Tissue culture
testis
Testosterone Desensitization
