摘要
Ｗｉｓｔａｒ大鼠１２６只随机分组，Ａｌｅｎ氏法２５０ｇ－ｃｍ致伤Ｔ１３～Ｌ１节段脊髓。应用三七总皂甙（ＰＮＳ）于伤后３０分钟，２小时，４小时，第二及第三个２４时分别腹腔注射给药；另设二甲亚砜（ＤＭＳＯ）对照组和空白对照组。８１只动物观察神经功能６周。结果显示脊髓损伤早期ＰＮＳ治疗组功能恢复有显著差异，（Ｐ值＜０．０１）；脊髓出血坏死较轻，周围白质大部分存留，毛细血管及少突胶质细胞增生，神经功能恢复与组织学结果相一致。提示ＰＮＳ虽不能完全阻止脊髓损伤后的灰质坏死，其调节微循环和减轻继发性病理损害的作用对白质的存活创造了条件，使脊髓的传导功能得以改善。
nehunderdandtwenty-sixWistarratswereusedtoobservetheefectsofpanaxnotoginsengsaponins(PNS)onearlyspinalcordinjury(SCI)atthelowerthoracicandupperlumbarlevelsresulted-withAlenstechnique.TheefectswerecomparedwiththatofDimethylSulfoxide(DMSO),aknownefectivedrugonthetreatmentofSCI,andwiththatofthecontrols.PNSwasinjectedintotheabdomi-nalcavityat30min,2hours,4hours,thesecondandthethird24hoursfolowingSCIindifferentgroupsandperiods.81ratswereobservedandneurologicalyevaluatedfor6weeks;asmalseriesofratsunderwentmorphologicexaminationbylightandelectronmicroscopes.TheresultsrevealedthattheanimalstreatedwithPNSexhibitedbeterclinicalimprovementthanthatofthecontrols(P<0.01)asevaluatedneurologicalywithTarlovscaleandincline-planetestinearlySCI.MorphologicfindingsofPNSgrouprevealedslighthemorrhage,necrosiswithsurvivalofthesurroundingwhitemat-terandproliferationofabundantcapilariesandoligodendrocytes.TheresultswascloselysimilarwiththosetreatedwithDMSO.AlthoughPNScouldnotpreventthenecrosisofthegreymaterfolowingspinalcordinjury,yetitseffectsontheimprovementofspinalcordmicrocirculationandmarkedde-creaseofsecondarypathologicaldamagehadprotectedthewhitematerinearlySCI.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1996年第6期384-387,共4页
Chinese Journal of Orthopaedics
关键词
脊髓损伤
截瘫
三七总皂甙
SpinalcordinjuryParaplegiaPanaxnotoginsengsaponinsRat
