摘要
目的:建立爪蟾卵母细胞双电极电压钳记录技术。方法:在去除滤泡膜的非洲爪蟾卵母细胞上,建立双电极电压钳记录技术,并使用该技术观察爪蟾卵母细胞表达的内源性递质门控性和电压门控性离子通道电流。结果:灌流液中分别加入NMDA受体、γ-氨基丁酸和烟碱受体激动剂,均未能在爪蟾卵母细胞上诱发出电流。去极化刺激仅可诱发出一外向电流。此电流的幅度随细胞外液中Ca2+浓度的增加而增大。当外液中无Ca2+或无C l-时,电流消失。此电流不被TEA所阻断,但能被MnC l2可逆性阻断。结论:成功建立了爪蟾卵母细胞双电极电压钳记录技术。去极化刺激在爪蟾卵母细胞上诱发出的外向电流为钙依赖性C l-电流。
Objective：To establish two-electrode voltage clamp recording technique for Xenopus laevis oocyte. Methods： The ligand-gated and voltage-gated ion channel currents expressed in X.laevis oocytes without follicles were observed using two-eletrode voltage-clamp techhique. Results： There were no visible currents induced in X.laevis oocytes when NMDA, γ-GABA and nicotine were added in the perfusion fluids, respectively. Membrane depolarization could evoke an outward current. The amplitude of currents increased when Ca^2＋ concentration in the extracellular fluids was raised. The currents disappeared in the extracellular solution without Ca^2＋ and/or Cl^-. The outward currents could be blocked by MnCl2, but not by TEA. Conclusion： The two-electrode voltage clamp recording technique for X. laevis oocytes is successfully established. The outward currents induced in X. laevis oocytes are Ca^2＋ -dependent Cl^- currents.
出处
《军事医学科学院院刊》
CSCD
北大核心
2005年第5期460-463,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家自然科学基金资助项目(2003CB515406
30371677
30472019)
关键词
爪蟾卵母细胞
内源性外向电流
双电极电压钳
Xenopus laevis oocyte
endogenous outward currents
two-electrode voltage clamp
