摘要
目的探讨p53和端粒酶逆转录酶(hTERT)在膀胱移行上皮癌的表达和作用。方法应用免疫组织化学方法和原位分子杂交检测膀胱移行上皮癌的组织学标本的p53蛋白和 hTERT mRNA表达,结合病理学和临床资料进行相关分析。结果 62例标本中p53阳性表达21 例(33．87％),hTERT阳性表达48例(77．42％)。p53的表达与肿瘤的病理学分级显著相关(P< 0．01),与肿瘤的复发显著相关(P<0．01)。hTERT的表达与肿瘤的分级、分期及复发无相关性。结论 p53基因和端粒酶参与了膀胱移行上皮癌的发生和进展。p53蛋白阳性表达有较高的肿瘤分级,并且更可能复发。hTERT可作为临床膀胱移行上皮癌的诊断指标之一。
Objective To investigate the expression and role of p53 and hTERT in bladder transitional cell carcinoma, Methods Pathologically diagnosed bladder transitional cell carcinoma were enrolled in this study. The immunohistochemistry was used to detect the expression of P53 and in sltu hybridization was used to detect hTERT mRNA. their immunostaining data was compared with clinical pathological outcomes. Results Among of the 62 specimen. P53 was expressed in 21 specimen （33.87 % ） and hTERT was detected in 48 specimen（77.42 % ）. The expression of p53 was positively associated with high-grade tumor （ P 〈 0.01 ） and recurrent tumor （ P 〈 0.01）. There was no significant difference between the expression of hTERT and tumor pathological outcomes. Conclusion p53 and Telomerase were involved in the genesis and progression in bladder transitional cell carcinoma. The expression of p53 was closely associated with high-grade tumors and recurrent tumors. The hTERT may be one of markers on diagnosis of bladder transitional cell carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第12期1557-1558,共2页
Chinese Journal of Experimental Surgery
