摘要
报告用ＨＬＡ相合的同胞异基困外周血造血干细胞移植治疗白血病３例，１例为ＣＭＬ－ＣＰ，１例为ＡＬＬ－ＣＲ１，１例为ＡＭＬ－Ｍ２ＣＲ１，预处理方案均为环磷酰胺６０ｍｇ／ｋｇ×２天，全身６０Ｃｏ照射４Ｇｙ×３天，供者于－５日起间步每日给予Ｇ－ＣＳＦ５μｇ／ｋｇ，－５日后即－１日用ＢａｘｅｒＣＳ３０００血细胞分离机分离单个核细胞（ＭＮＣ）其ＭＮＣ为７．７０、３．４４及２．４６×１０８／ｋｇ，ＬＤ３４＋细胞为２５．７及１９．２×１０６／ｋｇ，ＣＤ３４＋／ＣＤ３３－细胞为１．１１及０．５９×１０６／ｋｇ，ＣＤ３＋细胞为９４．９及１３６．８×１０６／ｋｇ，血循环量为１０升分离后给思考静脉输注（在ＴＢＩ４小时以后）．次日（０日）再分离一次ＭＮＣ输注给病人，认为Ａｌｌｏ－ＰＢＳＣＴ可以使造血细胞植入恢复得快，感染及出血的机会减少，抗菌素的使用天数减少，急性ＧＶＨＤ并不增多、对Ａｌｌｏ－ＰＢＳＣＴ的有关问题讨论。
We reported three patients with leukemia transplanted with allogeneic peripheralblood stem cells of HLA matchcd siblings. They are a man with CML in chronic phasc, a femalewith ALL in CR1 and a man with AML in CR1. All of them were transplanted with HLAidentical siblings' Allo-PBSC. After conditioned with CTX 60mg/kg×2 and TBI 4.0Gy×3,themononouclear cell which were collected by leukophereses,(Baxter CS3000 101 ) were infusedwithout cryoprcscrvation for two times. The NMC infused, in cascs 1,2 and 3 were 7.70,3.44and 2.46×108/kg respectively. The CD34+, CD34+/CD33- and CD3+ cells infused were 78.3and 21.1,30.1 and 20.0,94.9 and 136.8 ×106/kg respectively in case 2 and case 3.Thehacmopoiesis recovery was much faster than that of the BMT group. Acute GVHD of grade Ⅱ、Ⅰand Ⅲ occured in cases 1.2 and 3 respcctivcly.All of them wcre controlled by prednisolone.We reviewed some reports of Allo-PBSCT and concluded that Allo-PBSCT are the alternativesource of stem cells of transplantation. Patients with Allo-PBSCT has a faster recovery ofleukocy te and platelet than with bone marrow transp lantation, Patients with Allo-PBSCT haveless risk of infection and hemorrhage, and reduced thC need of blood prcduct support.
出处
《白血病》
1996年第2期66-69,共4页
